Počet záznamů: 1
Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo
- 1.
SYSNO ASEP 0556620 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Coating Ti6Al4V implants with nanocrystalline diamond functionalized with BMP-7 promotes extracellular matrix mineralization in vitro and faster osseointegration in vivo Tvůrce(i) Němčáková, Ivana (FGU-C) RID, ORCID
Litvinec, Andrej (FGU-C)
Mandys, V. (CZ)
Potocký, Štěpán (FZU-D) RID, ORCID
Plencner, Martin (FGU-C) RID, ORCID
Doubková, Martina (FGU-C) ORCID, RID
Naňka, O. (CZ)
Olejníčková, Veronika (FGU-C) RID, ORCID, SAI
Šaňková, Barbora (FGU-C) RID, ORCID
Bartoš, M. (CZ)
Ukraintsev, E. (CZ)
Babčenko, O. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Kromka, Alexander (FZU-D) RID, ORCID, SAI
Rezek, B. (CZ)
Sedmera, David (FGU-C) RID, ORCID, SAICelkový počet autorů 16 Číslo článku 5264 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 12, č. 1 (2022)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova alkaline-phosphatase activity ; human osteogenic protein-1 ; mesenchymal stem-cells ; bone-formation ; surface functionalization ; antiadhesive properties ; metallic implants ; titanium implants ; dental implants Obor OECD Biomaterials (as related to medical implants, devices, sensors) Vědní obor RIV – spolupráce Fyzikální ústav - Chirurgie vč. transplantologie CEP GA21-06065S GA ČR - Grantová agentura ČR NV15-32497A GA MZd - Ministerstvo zdravotnictví LM2018110 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_019/0000760 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CzechNanoLab - 90110 - Vysoké učení technické v Brně Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; FZU-D - RVO:68378271 UT WOS 000774204500044 EID SCOPUS 85127270573 DOI https://doi.org/10.1038/s41598-022-09183-z Anotace The present study investigates the effect of an oxidized nanocrystalline diamond (O-NCD) coating functionalized with bone morphogenetic protein 7 (BMP-7) on human osteoblast maturation and extracellular matrix mineralization in vitro and on new bone formation in vivo. The chemical structure and the morphology of the NCD coating and the adhesion, thickness and morphology of the superimposed BMP-7 layer have also been assessed. The material analysis proved synthesis of a conformal diamond coating with a fine nanostructured morphology on the Ti6Al4V samples. The homogeneous nanostructured layer of BMP-7 on the NCD coating created by a physisorption method was confirmed by AFM. The osteogenic maturation of hFOB 1.19 cells in vitro was only slightly enhanced by the O-NCD coating alone without any increase in the mineralization of the matrix. Functionalization of the coating with BMP-7 resulted in more pronounced cell osteogenic maturation and increased extracellular matrix mineralization. Similar results were obtained in vivo from micro-CT and histological analyses of rabbit distal femurs with screws implanted for 4 or 12 weeks. While the O-NCD-coated implants alone promoted greater thickness of newly-formed bone in direct contact with the implant surface than the bare material, a further increase was induced by BMP-7. It can be therefore concluded that O-NCD coating functionalized with BMP-7 is a promising surface modification of metallic bone implants in order to improve their osseointegration. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://doi.org/10.1038/s41598-022-09183-z
Počet záznamů: 1