Počet záznamů: 1
Giardia duodenalis in a clinically healthy population of captive zoo chimpanzees: Rapid antigen testing, diagnostic real-time PCR and faecal microbiota profiling
- 1.
SYSNO ASEP 0556445 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Giardia duodenalis in a clinically healthy population of captive zoo chimpanzees: Rapid antigen testing, diagnostic real-time PCR and faecal microbiota profiling Tvůrce(i) Willenborg, C. M. (AU)
Červená, Barbora (UBO-W) RID, ORCID, SAI
Thompson, P. (AU)
Rosario, E. (AU)
Ruaux, C. (AU)
Vogelnest, L. (AU)
Šlapeta, J. (AU)Celkový počet autorů 7 Zdroj.dok. International Journal for Parasitology: Parasites and Wildlife - ISSN 2213-2244
Roč. 17, April (2022), s. 308-318Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. AU - Austrálie Klíč. slova Giardiasis ; Diagnostics ; Zoonosis ; Zoo animals ; Parasite ; Commensal ; Microbiome Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína Obor OECD Veterinary science CEP EF16_027/0008027 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UBO-W - RVO:68081766 UT WOS 000793179800002 EID SCOPUS 85126921463 DOI 10.1016/j.ijppaw.2022.03.007 Anotace Giardia duodenalis is one of the most common intestinal parasites of humans, with a worldwide distribution. Giardia duodenalis has been reported in both wild and captive populations of non-human primates, namely chimpanzees. In this study we investigated an entire troop of clinically healthy chimpanzees (n = 21) for the presence of G. duodenalis and its association with faecal microbiota profile. Faecal samples (n = 26) were collected from the chimpanzee exhibit from a zoo in Sydney, Australia. Diagnosis of G. duodenalis was made using a Rapid Antigen Test (RAT) as a point-of-care-test and compared to a reference standard real-time PCR test. Approximately half of the chimpanzee faecal samples tested positive for G. duodenalis by both RAT (13/26, 50%) and real-time PCR (14/26, 53.85%). The RAT sensitivity was 85.7% (95% CI: 63.8%–96%) and specificity was 91.7% (95% CI: 68.3%–99%) when compared to the in-house real-time PCR. Genotyping of the samples revealed the presence of zoonotic assemblage B. Microscopic analysis revealed the presence of Troglodytella spp. (14/26), Balantioides sp. (syn. Balantidium sp.) (8/26) as well as Entamoeba spp. (3/26). Microbiota profile based on 16S rRNA gene sequencing revealed that the community was significantly different between G. duodenalis positive and negative samples if RAT results were taken into an account, but not real-time PCR diagnostics results. Proteobacteria and Chloroflexi were the significant features in the dataset that separated G. duodenalis positive and negative samples using LEfSe analysis. Being able to rapidly test for G. duodenalis in captive populations of primates assists in point-of-care diagnostics and may better identify animals with subclinical disease. Under the investigated conditions of the zoo setting, however, presence of G. duodenalis either detected by RAT or real-time PCR was not associated with clinically apparent disease in captive chimpanzees. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2023 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2213224422000268?via%3Dihub
Počet záznamů: 1