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Intestinal mycobiome associated with diagnosis of inflammatory bowel disease based on tissue biopsies

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    SYSNO ASEP0555931
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevIntestinal mycobiome associated with diagnosis of inflammatory bowel disease based on tissue biopsies
    Tvůrce(i) Cimická, Jana (FGU-C)
    Riegert, J. (CZ)
    Kavková, M. (CZ)
    Černá, K. (CZ)
    Celkový počet autorů4
    Číslo článkumyab076
    Zdroj.dok.Medical Mycology - ISSN 1369-3786
    Roč. 60, č. 1 (2022)
    Poč.str.10 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovamycobiome ; FFPE samples ; ulcerative colitis ; Crohn's disease ; NGS
    Obor OECDMicrobiology
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000763632600001
    EID SCOPUS85123651543
    AnotaceAnalysis of mycobiome from formalin-fixed, paraffin-embedded (FFPE) biopsies should preferentially detect only fungi which are actually present in the intestine wall, in contrast to stool samples, which are limited by the diet composition. Next generation sequencing provides the advantage of analyzing many species from a single sample. Consequently, canonical correspondence analysis divided fungal genera present in FFPE intestinal tissues into three well-defined experimental groups (negative controls NC, Crohn's disease CD, ulcerative colitis UC). Simultaneously, the analysis showed that particular fungal genera are associated with these experimental groups and several fungal genera occurred in all experimental groups equally. Our results also showed a noticeable increase of Ascomycota proportion from NC, through CD to UC. Fungal genera Malassezia, Cladosporium and Toninia occurred in all experimental groups assuming that they are common components of the intestinal mycobiome. Other fungal genera found only in the NC experimental group were non-pathogenic and might bring some benefits. In contrast, CD and UC samples were characterized by an accumulation of genera with inhibitive effects on growth of other fungal genera and the presence of opportunistic pathogens. Furthermore, a decrease in the fungal genus Malassezia in inflammatory tissues was observed, Specifically, the UC experimental group showed a connection between the presence of Candida and seven time's lower amounts of Malassezia (compared to amounts found in NC). The CD experimental group was characterized by the simultaneous presence of Engyodontium album with Lecanicillium, and indicates a possible pathogenic effect of Ramularia in disease development. Lay summary Mycobiome analysis of formalin-fixed and paraffin-embedded biopsies may highlight actual fungal genera composition in the intestinal wall. Interestingly, experimental groups of Crohn's disease and ulcerative colitis clearly differed by structure of their mycobiomes.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2023
    Elektronická adresahttps://doi.org/10.1093/mmy/myab076
Počet záznamů: 1