Počet záznamů: 1
Influence of Biomimetically Mineralized Collagen Scaffolds on Bone Cell Proliferation and Immune Activation
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SYSNO ASEP 0555930 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Influence of Biomimetically Mineralized Collagen Scaffolds on Bone Cell Proliferation and Immune Activation Tvůrce(i) Bačáková, Lucie (FGU-C) RID, ORCID
Novotná, Katarína (FGU-C)
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Musílková, Jana (FGU-C) RID, ORCID
Slepička, P. (CZ)
Beran, M. (CZ)Celkový počet autorů 6 Číslo článku 602 Zdroj.dok. Polymers. - : MDPI
Roč. 14, č. 3 (2022)Poč.str. 26 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova biopolymers ; nature-derived polymers ; porous scaffolds ; mechanical properties ; elemental composition ; regenerative medicine ; cell adhesion ; cell growth ; cell differentiation ; inflammatory activation Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP NU20-08-00208 GA MZd - Ministerstvo zdravotnictví LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000756296200001 EID SCOPUS 85124190525 DOI 10.3390/polym14030602 Anotace Collagen, as the main component of connective tissue, is frequently used in various tissue engineering applications. In this study, porous sponge-like collagen scaffolds were prepared by freeze-drying and were then mineralized in a simulated body fluid. The mechanical stability was similar in both types of scaffolds, but the mineralized scaffolds (MCS) contained significantly more calcium, magnesium and phosphorus than the unmineralized scaffolds (UCS). Although the MCS contained a lower percentage (~32.5%) of pores suitable for cell ingrowth (113-357 mu m in diameter) than the UCS (~70%), the number of human-osteoblast-like MG-63 cells on days 1, 3 and 7 after seeding was higher on MCS than on UCS, and the cells penetrated deeper into the MCS. The cell growth in extracts prepared by eluting the scaffolds for 7 days in a cell culture medium was also markedly higher in the MCS extracts, as indicated by real-time monitoring in the sensory xCELLigence system for 7 days. From this point of view, MCS are more promising for bone tissue engineering than UCS. However, MCS evoked a more pronounced inflammatory response than UCS, as indicated by the production of tumor necrosis factor-alpha (TNF-alpha) in macrophage-like RAW 264.7 cells in cultures on these scaffolds. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/2073-4360/14/3/602
Počet záznamů: 1