Počet záznamů: 1

Nanoscale analysis of nuclear phosphatidylinositol phosphate distribution between nucleaoplasm and nuclear speckles

SYSNO ASEP	0555748
Druh ASEP	O - Ostatní výsledky
Zařazení RIV	O - Ostatní
Název	Nanoscale analysis of nuclear phosphatidylinositol phosphate distribution between nucleaoplasm and nuclear speckles
Tvůrce(i)	Hoboth, Peter (UMG-J)_ORCID Sztacho, Martin (UMG-J)_ORCID Šebesta, O. (CZ) Hozák, Pavel (UMG-J)_{RID, ORCID}
Rok vydání	2021
Jazyk dok.	eng - angličtina
Země vyd.	CZ - Česká republika
Klíč. slova	single-molecule localization microscopy ; nuclear phosphatidylinositol phosphates ; nucleoplasm ; RNA polymerase II transcription
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Cell biology
CEP	LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA19-05608S GA ČR - Grantová agentura ČR
	GA18-19714S GA ČR - Grantová agentura ČR
	LTC19048 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	LTC20024 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Institucionální podpora	UMG-J - RVO:68378050
Anotace	Single-molecule localization microscopy (SMLM) provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids. However, the role of the nuclear lipids in the establishment of the functional nuclear architecture, apart from the nuclear envelope, has been neglected. Nevertheless, the roles in gene expression of the nuclear lipids and phosphatidylinositol phosphates (PIPs) in particular started to emerge. Therefore, we implemented and optimized the SMLM-based approach for the quantitative evaluation of the nuclear PIP distribution while preserving the context of nuclear architecture. We have quantitatively characterized the spatial distribution of nuclear phosphatidylinositol 4,5- and 3,4-bisphosphate (PI(4,5)P2 and PI(3,4)P2, resp.) and showed that PI(4,5)P2 and PI(3,4)P2 localize within matrix of the nuclear speckle marker SON and in the nucleoplasmic foci. Moreover, we found PI(4,5)P2 and PI(3,4)P2 in the close proximity with the subset of RNAPII foci either in the nucleoplasm or at nuclear speckles. We started to investigate the cross-talk between nucleoplasmic and nuclear speckle-associated PI(4,5)P2 and PI(3,4)P2 pools and their possible roles in the regulation of RNAPII transcription. Our preliminary data suggest that upon transcription inhibition PI(4,5)P2 and PI(3,4)P2 accumulate within nuclear speckles. Therefore, nuclear speckles could play a role in the buffering of the nuclear PIP levels and thereby possibly regulate RNAPII transcription.
Pracoviště	Ústav molekulární genetiky
Kontakt	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Rok sběru	2022

Počet záznamů: 1