Počet záznamů: 1
Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus
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SYSNO ASEP 0555020 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus Tvůrce(i) Macháčová, Simona (UMG-J)
Chmelová, Helena (UMG-J)
Vávrová, Anna (UMG-J)
Kozmik, Zbyněk (UMG-J) RID
Kozmiková, Iryna (UMG-J) RIDCelkový počet autorů 5 Číslo článku 702986 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 9, July (2021)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova amphioxus ; light sheet microscopy ; clearing technique ; whole mount immunohistochemistry ; photoreceptor ; acetylated tubulin ; melanopsin Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP GA20-25377S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000682949500001 DOI 10.3389/fcell.2021.702986 Anotace Cephalochordates (amphioxi or lancelets) are representatives of the most basally divergent group of the chordate phylum. Studies of amphioxus development and anatomy hence provide a key insight into vertebrate evolution. More widespread use of amphioxus in the evo-devo field would be greatly facilitated by expanding the methodological toolbox available in this model system. For example, evo-devo research on amphioxus requires deep understanding of animal anatomy. Although conventional confocal microscopy can visualize transparent amphioxus embryos and early larvae, the imaging of later developmental stages is problematic because of the size and opaqueness of the animal. Here, we show that light sheet microscopy combined with tissue clearing methods enables exploration of large amphioxus specimens while keeping the surface and the internal structures intact. We took advantage of the phenomenon of autofluorescence of amphioxus larva to highlight anatomical details. In order to investigate molecular markers at the single-cell level, we performed antibody-based immunodetection of melanopsin and acetylated-alpha-tubulin to label rhabdomeric photoreceptors and the neuronal scaffold. Our approach that combines light sheet microscopy with the clearing protocol, autofluorescence properties of amphioxus, and antibody immunodetection allows visualizing anatomical structures and even individual cells in the 3D space of the entire animal body. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2022 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fcell.2021.702986/full
Počet záznamů: 1