Počet záznamů: 1
An ABC transporter Wzm-Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria
- 1.
SYSNO ASEP 0554945 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název An ABC transporter Wzm-Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria Tvůrce(i) Savková, K. (SK)
Huszár, S. (SK)
Baráth, P. (SK)
Pakanová, Z. (SK)
Kozmon, S. (SK)
Vancová, Marie (BC-A) RID, ORCID
Tesařová, Martina (BC-A) ORCID
Blasko, J. (SK)
Kalinak, M. (SK)
Singh, V. (ZA)
Korduláková, J. (SK)
Mikušová, K. (SK)Celkový počet autorů 12 Číslo článku e2023663118 Zdroj.dok. Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 118, č. 17 (2021)Poč.str. 10 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova lipopolysaccharide o-antigens ; cell-wall ; klebsiella-pneumoniae ; escherichia-coli ; linkage region ; chain-length ; biosynthesis ; arabinogalactan ; tuberculosis ; lipoarabinomannan ; mycobacterium ; cell wall ; transport ; galactan Vědní obor RIV EA - Morfologické obory a cytologie Obor OECD Cell biology CEP LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000648438500019 EID SCOPUS 85105348097 DOI 10.1073/pnas.2023663118 Anotace Mycobacterium tuberculosis, one of the deadliest pathogens in human history, is distinguished by a unique, multilayered cell wall, which offers the bacterium a high level of protection from the attacks of the host immune system. The primary structure of the cell wall core, composed of covalently linked peptidoglycan, branched heteropolysaccharide arabinogalactan, and mycolic acids, is well known, and numerous enzymes involved in the biosynthesis of its components are characterized. The cell wall biogenesis takes place at both cytoplasmic and periplasmic faces of the plasma membrane, and only recently some of the specific transport systems translocating the metabolic intermediates between these two compartments have been characterized [M. Jackson, C. M. Stevens, L. Zhang, H. I. Zgurskaya, M. Niederweis, Chem. Rev., 10.1021/acs.chemrev.0c00869 (2020)]. In this work, we use CRISPR interference methodology in Mycobacterium smegmatis to functionally characterize an ATP binding cassette (ABC) transporter involved in the translocation of galactan precursors across the plasma membrane. We show that genetic knockdown of the transmembrane subunit of the transporter results in severe morphological changes and the accumulation of an aberrantly long galactan precursor. Based on similarities with structures and functions of specific O-antigen ABC transporters of gram-negative bacteria [C. Whitfield, D. M. Williams, S. D. Kelly, J. Biol. Chem. 295, 10593-10609 (2020)], we propose a model for coupled synthesis and export of the galactan polymer precursor in mycobacteria. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.pnas.org/doi/full/10.1073/pnas.2023663118
Počet záznamů: 1