Počet záznamů: 1
Incidence of Phage Capsid Organization on the Resistance to High Energy Proton Beams
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SYSNO ASEP 0554843 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Incidence of Phage Capsid Organization on the Resistance to High Energy Proton Beams Tvůrce(i) De Plano, L. M. (IT)
Silipigni, L. (IT)
Torrisi, L. (IT)
Torrisi, A. (IT)
Cutroneo, Mariapompea (UJF-V) ORCID, RID, SAI
Havránek, Vladimír (UJF-V) RID, SAI, ORCID
Macková, Anna (UJF-V) RID, ORCID, SAI
Zammuto, V. (IT)
Gugliandolo, C. (IT)
Rizzo, M. G. (IT)
Guglielmino, S. (IT)
Franco, D. (IT)Celkový počet autorů 12 Číslo článku 988 Zdroj.dok. Applied Sciences-Basel. - : MDPI
Roč. 12, č. 3 (2022)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova M13 bacteriophages ; engineered phage clones ; proton beam radiation ; resistance to particles radiation Obor OECD Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect) CEP EF16_013/0001812 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CANAM II - 90056 - Ústav jaderné fyziky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora UJF-V - RVO:61389005 UT WOS 000759884100001 EID SCOPUS 85123117213 DOI 10.3390/app12030988 Anotace The helical geometry of virus capsid allows simple self-assembly of identical protein subunits with a low request of free energy and a similar spiral path to virus nucleic acid. Consequently, small variations in protein subunits can affect the stability of the entire phage particle. Previously, we observed that rearrangement in the capsid structure of M13 engineered phages affected the resistance to UV-C exposure, while that to H2O2 was mainly ascribable to the amino acids' sequence of the foreign peptide. Based on these findings, in this work, the resistance to accelerated proton beam exposure (5.0 MeV energy) of the same phage clones was determined at different absorbed doses and dose rates. Then, the number of viral particles able to infect and replicate in the natural host, Escherichia coli F+, was evaluated. By comparing the results with the M13 wild-type vector (pC89), we observed that 12III1 phage clones, with the foreign peptide containing amino acids favorable to carbonylation, exhibited the highest reduction in phage titer associated with a radiation damage (RD) of 35 x 10(-3)/Gy at 50 dose Gy. On the other hand, P9b phage clones, containing amino acids unfavorable to carbonylation, showed the lowest reduction with an RD of 4.83 x 10(-3)/Gy at 500 dose Gy. These findings could improve the understanding of the molecular mechanisms underlying the radiation resistance of viruses Pracoviště Ústav jaderné fyziky Kontakt Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Rok sběru 2023 Elektronická adresa https://doi.org/10.3390/app12030988
Počet záznamů: 1