Počet záznamů: 1
Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging
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SYSNO ASEP 0554418 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging Tvůrce(i) Hlaváček, Antonín (UIACH-O) ORCID
Farka, Z. (CZ)
Mickert, M. J. (SE)
Kostiv, Uliana (UMCH-V) RID
Brandmeier, J. C. (DE)
Horák, Daniel (UMCH-V) RID, ORCID
Skládal, P. (CZ)
Foret, František (UIACH-O) RID, ORCID
Gorris, H. H. (DE)Celkový počet autorů 9 Zdroj.dok. Nature Protocols. - : Nature Publishing Group - ISSN 1754-2189
Roč. 17, č. 4 (2022), s. 1028-1072Poč.str. 45 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova photon upconversion nanoparticle ; biomarkers ; surface modification Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP GA21-03156S GA ČR - Grantová agentura ČR GA21-04420S GA ČR - Grantová agentura ČR LTAB19011 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UIACH-O - RVO:68081715 ; UMCH-V - RVO:61389013 UT WOS 000757750800001 EID SCOPUS 85124960324 DOI 10.1038/s41596-021-00670-7 Anotace The detection of cancer biomarkers in histological samples and blood is of paramount importance for clinical diagnosis. Current methods are limited in terms of sensitivity, hindering early detection of disease. We have overcome the shortcomings of currently available staining and fluorescence labeling methods by taking an integrative approach to establish photon-upconversion nanoparticles (UCNP) as a powerful platform for cancer detection. These nanoparticles are readily synthesized in different sizes to yield efficient and tunable short-wavelength light emission under near-infrared excitation, which eliminates optical background interference of the specimen. Here we present a protocol for the synthesis of UCNPs by high-temperature co-precipitation or seed-mediated growth by thermal decomposition, surface modification by silica or poly(ethylene glycol) that renders the particles resistant to nonspecific binding, and the conjugation of streptavidin or antibodies for biological detection. To detect blood-based biomarkers, we present an upconversion-linked immunosorbent assay for the analog and digital detection of the cancer marker prostate-specific antigen. When applied to immunocytochemistry analysis, UCNPs enable the detection of the breast cancer marker human epidermal growth factor receptor 2 with a signal-to-background ratio 50-fold higher than conventional fluorescent labels. UCNP synthesis takes 4.5 d, the preparation of the antibody–silica–UCNP conjugate takes 3 d, the streptavidin–poly(ethylene glycol)–UCNP conjugate takes 2–3 weeks, upconversion-linked immunosorbent assay takes 2–4 d and immunocytochemistry takes 8–10 h. The procedures can be performed after standard laboratory training in nanomaterials research. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2023 Elektronická adresa http://hdl.handle.net/11104/0329128
Počet záznamů: 1