Počet záznamů: 1
Tick defensin gamma-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency
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SYSNO ASEP 0554258 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tick defensin gamma-core reduces Fusarium graminearum growth and abrogates mycotoxins production with high efficiency Tvůrce(i) Leannec-Rialland, V. (FR)
Cabezas-Cruz, A. (FR)
Atanasova, V. (FR)
Chereau, S. (FR)
Ponts, N. (FR)
Tonk, M. (DE)
Vilcinskas, A. (DE)
Ferrer, N.G. (AT)
Valdés, James J. (BC-A) RID, ORCID
Richard-Forget, F. (FR)Celkový počet autorů 10 Číslo článku 7962 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova antifungal plant defensins ; force-field ; protein ; dynamics ; automation ; parameters ; interface ; peptides ; acid ; nad1 Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000640434400095 EID SCOPUS 85104248635 DOI 10.1038/s41598-021-86904-w Anotace Fusarium graminearum is a major fungal pathogen affecting crops of worldwide importance. F. graminearum produces type B trichothecene mycotoxins (TCTB), which are not fully eliminated during food and feed processing. Therefore, the best way to minimize TCTB contamination is to develop prevention strategies. Herein we show that treatment with the reduced form of the gammacore of the tick defensin DefMT3, referred to as TickCore3 (TC3), decreases F. graminearum growth and abrogates TCTB production. The oxidized form of TC3 loses antifungal activity, but retains anti-mycotoxin activity. Molecular dynamics show that TC3 is recruited by specific membrane phospholipids in F. graminearum and that membrane binding of the oxidized form of TC3 is unstable. Capping each of the three cysteine residues of TC3 with methyl groups reduces its inhibitory efficacy. Substitutions of the positively-charged residues lysine (Lys) 6 or arginine 7 by threonine had the highest and the lesser impact, respectively, on the anti-mycotoxin activity of TC3. We conclude that the binding of linear TC3 to F. graminearum membrane phospholipids is required for the antifungal activity of the reduced peptide. Besides, Lys6 appears essential for the anti-mycotoxin activity of the reduced peptide. Our results provide foundation for developing novel and environment-friendly strategies for controlling F. graminearum. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.nature.com/articles/s41598-021-86904-w
Počet záznamů: 1