Počet záznamů: 1
Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy
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SYSNO ASEP 0553484 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Size and Fluorescence Properties of Algal Photosynthetic Antenna Proteins Estimated by Microscopy Tvůrce(i) Crepin, Aurelia (MBU-M) ORCID
Belgio, Erica (MBU-M)
Šedivá, Barbora (MBU-M)
Trsková, Eliška (MBU-M)
Cunill-Semanat, Edel (MBU-M)
Kaňa, Radek (MBU-M) RID, ORCIDČíslo článku 778 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 2 (2022)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova light-harvesting-complex ; photosystem-ii ; chromera-velia ; membrane ; supercomplex ; acclimation ; plants ; photosynthesis ; antenna proteins ; light-harvesting ; fluorescence correlation spectroscopy ; protein diffusion ; microscopy ; protein oligomerization ; Chromera velia Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA19-11494S GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000746412300001 EID SCOPUS 85122491782 DOI 10.3390/ijms23020778 Anotace Antenna proteins play a major role in the regulation of light-harvesting in photosynthesis. However, less is known about a possible link between their sizes (oligomerization state) and fluorescence intensity (number of photons emitted). Here, we used a microscopy-based method, Fluorescence Correlation Spectroscopy (FCS), to analyze different antenna proteins at the particle level. The direct comparison indicated that Chromera Light Harvesting (CLH) antenna particles (isolated from Chromera velia) behaved as the monomeric Light Harvesting Complex II (LHCII) (from higher plants), in terms of their radius (based on the diffusion time) and fluorescence yields. FCS data thus indicated a monomeric oligomerization state of algal CLH antenna (at our experimental conditions) that was later confirmed also by biochemical experiments. Additionally, our data provide a proof of concept that the FCS method is well suited to measure proteins sizes (oligomerization state) and fluorescence intensities (photon counts) of antenna proteins per single particle (monomers and oligomers). We proved that antenna monomers (CLH and LHCIIm) are more ´quenched´ than the corresponding trimers. The FCS measurement thus represents a useful experimental approach that allows studying the role of antenna oligomerization in the mechanism of photoprotection. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/1422-0067/23/2/778
Počet záznamů: 1