Počet záznamů: 1
Tandem gene amplification restores photosystem II accumulation in cytochrome b(559) mutants of cyanobacteria
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SYSNO ASEP 0553481 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tandem gene amplification restores photosystem II accumulation in cytochrome b(559) mutants of cyanobacteria Tvůrce(i) Chiu, Y. (TW)
Fu, H. (TW)
Skotnicová, Petra (MBU-M) ORCID, RID
Lin, K. (TW)
Komenda, Josef (MBU-M) RID, ORCID
Chu, H. (TW)Zdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 233, č. 2 (2022), s. 766-780Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova site-directed mutagenesis ; pcc 6803 mutants ; photosynthetic growth ; electron flow ; axial-ligands ; synechocystis ; complex ; protein ; repair ; light ; cyanobacterium ; cytochrome b(559) ; photosynthesis ; photosystem II (PSII) ; tandem gene amplification Obor OECD Microbiology Vědní obor RIV – spolupráce Mikrobiologický ústav - Mikrobiologie, virologie CEP GX19-29225X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000712906000001 EID SCOPUS 85118240989 DOI 10.1111/nph.17785 Anotace Cytochrome (Cyt) b(559) is a key component of the photosystem II complex (PSII) that is essential for its proper functioning and assembly. Site-directed mutants of the model cyanobacterium Synechocystis sp. PCC6803 with mutated heme axial ligands of Cyt b(559) have little PSII and are therefore unable to grow photoautotrophically. Here we describe two types of Synechocystis autotrophic transformants that retained the same mutations in Cyt b(559) but are able to accumulate PSII and grow photoautotrophically. Whole-genome sequencing revealed that all of these autotrophic transformants carried a variable number of tandem repeats (from 5 to 15) of chromosomal segments containing the psbEFLJ operon. RNA-seq analysis showed greatly increased transcript levels of the psbEFLJ operon in these autotrophic transformants. Multiple copies of the psbEFLJ operon in these transformants were only maintained during autotrophic growth, while its copy numbers gradually decreased under photoheterotrophic conditions. Two-dimensional PAGE analysis of membrane proteins revealed a strong deficiency in PSII complexes in the Cyt b(559) mutants that was reversed in the autotrophic transformants. These results illustrate how tandem gene amplification restores PSII accumulation and photoautotrophic growth in Cyt b(559) mutants of cyanobacteria, and may serve as an important adaptive mechanism for cyanobacterial survival. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17785
Počet záznamů: 1