Počet záznamů: 1
Anaesthetic diethyl ether impairs long-distance electrical and jasmonate signaling in Arabidopsis thaliana
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SYSNO ASEP 0553376 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Anaesthetic diethyl ether impairs long-distance electrical and jasmonate signaling in Arabidopsis thaliana Tvůrce(i) Jakšová, J. (CZ)
Rác, M. (CZ)
Bokor, B. (SK)
Petřík, Ivan (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Reichelt, M. (DE)
Mithöfer, A. (DE)
Pavlovič, A. (CZ)Celkový počet autorů 8 Zdroj.dok. Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428
Roč. 169, DEC (2021), s. 311-321Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. FR - Francie Klíč. slova Anaesthesia ; Arabidopsis thaliana ; Calcium ; Electrical signal ; Heat wounding ; Jasmonic acid Obor OECD Biochemistry and molecular biology CEP EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000725594000006 EID SCOPUS 85119491875 DOI 10.1016/j.plaphy.2021.11.019 Anotace General volatile anaesthetics (GVA) inhibit electrical signal propagation in animal neurons. Although plants do not have neurons, they generate and propagate electrical signals systemically from a local damaged leaf to neighbouring leaves. This systemic electrical signal propagation is mediated by ligand-gated glutamate receptor-like (GLR) channels. Here, we investigated the effect of GVA diethyl ether on the systemic electrical and further downstream responses in Arabidopsis thaliana. We monitored electrical signals, cytoplasmic Ca2+ level ([Ca2+]cyt), ultra-weak photon emission, amino acid contents, phytohormone response as well as gene expression in response to heat wounding during diethyl ether anaesthesia. We found complete suppression of electrical and [Ca2+]cyt signal propagation from damaged leaf to neighbouring systemic leaves upon diethyl ether treatment. Concomitantly, jasmonates (JAs) did not accumulate and expression of JA-responsive genes (AOS, OPR3, JAZ10) was not detected in systemic leaves. However local damaged leaves still showed increased [Ca2+]cyt and accumulated high level of JAs and JA-inducible transcripts. An exogenously added GLR ligand, L-glutamate, was not able to trigger Ca2+ wave in etherized plants indicating that GLRs are targeted by diethyl ether, but not specifically. The fact that GVA inhibit electrical signal propagation not only in animals but also in plants is intriguing. However, the cellular response is completely blocked only in systemic leaves, the local damaged leaf still senses damaging stimuli. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.1016/j.plaphy.2021.11.019
Počet záznamů: 1