Počet záznamů: 1
Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation
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SYSNO ASEP 0553280 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Healing and Angiogenic Properties of Collagen/Chitosan Scaffolds Enriched with Hyperstable FGF2-STAB(R) Protein: In Vitro, Ex Ovo and In Vivo Comprehensive Evaluation Tvůrce(i) Vojtová, L. (CZ)
Pavliňáková, V. (CZ)
Muchova, J. (CZ)
Kacvinská, K. (CZ)
Brtníková, J. (CZ)
Knoz, M. (CZ)
Lipový, B. (CZ)
Faldyna, M. (CZ)
Gopfert, E. (CZ)
Holoubek, J. (CZ)
Pavlovský, Z. (CZ)
Vicenová, M. (CZ)
Hefka Blahnová, Veronika (UEM-P)
Hearnden, V. (GB)
Filová, Eva (UEM-P) RID, ORCIDČíslo článku 590 Zdroj.dok. Biomedicines. - : MDPI
Roč. 9, č. 6 (2021)Poč.str. 27 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova collagen ; chitosan ; scaffold ; FGF2 ; skin regeneration ; tissue engineering Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000665273200001 EID SCOPUS 85107137204 DOI 10.3390/biomedicines9060590 Anotace Wound healing is a process regulated by a complex interaction of multiple growth factors including fibroblast growth factor 2 (FGF2). Although FGF2 appears in several tissue engineered studies, its applications are limited due to its low stability both in vitro and in vivo. Here, this shortcoming is overcome by a unique nine-point mutant of the low molecular weight isoform FGF2 retaining full biological activity even after twenty days at 37 degrees C. Crosslinked freeze-dried 3D porous collagen/chitosan scaffolds enriched with this hyper stable recombinant human protein named FGF2-STAB(R) were tested for in vitro biocompatibility and cytotoxicity using murine 3T3-A31 fibroblasts, for angiogenic potential using an ex ovo chick chorioallantoic membrane assay and for wound healing in vivo with 3-month old white New Zealand rabbits. Metabolic activity assays indicated the positive effect of FGF2-STAB(R) already at very low concentrations (0.01 mu g/mL). The angiogenic properties examined ex ovo showed enhanced vascularization of the tested scaffolds. Histological evaluation and gene expression analysis by RT-qPCR proved newly formed granulation tissue at the place of a previous skin defect without significant inflammation infiltration in vivo. This work highlights the safety and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB(R) protein. Moreover, these sponges could be used as scaffolds for growing cells for dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2227-9059/9/6/590
Počet záznamů: 1