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The Toxic Effects of Ppz1 Overexpression Involve Nha1-Mediated Deregulation of K+ and H+ Homeostasis

SYSNO ASEP	0551602
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The Toxic Effects of Ppz1 Overexpression Involve Nha1-Mediated Deregulation of K+ and H+ Homeostasis
Tvůrce(i)	Albacar, M. (ES) Sácká, Lenka (FGU-C) Calafí, C. (ES) Velázquez, Diego (FGU-C) Casamayor, A. (ES) Ariňo, J. (ES) Zimmermannová, Olga (FGU-C)_{RID, ORCID}
Číslo článku	1010
Zdroj.dok.	Journal of Fungi. - : MDPI Roč. 7, č. 12 (2021)
Poč.str.	17 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	Ppz1 phosphatase ; cation homeostasis ; Nha1 ; intracellular pH ; K+ transport ; Saccharomyces cerevisiae
Obor OECD	Biochemistry and molecular biology
CEP	GA21-08985S GA ČR - Grantová agentura ČR
	LTC20006 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000737637700001
EID SCOPUS	85123016229
DOI	10.3390/jof7121010
Anotace	The alteration of the fine-tuned balance of phospho/dephosphorylation reactions in the cell often results in functional disturbance. In the yeast Saccharomyces cerevisiae, the overexpression of Ser/Thr phosphatase Ppz1 drastically blocks cell proliferation, with a profound change in the transcriptomic and phosphoproteomic profiles. While the deleterious effect on growth likely derives from the alteration of multiple targets, the precise mechanisms are still obscure. Ppz1 is a negative effector of potassium influx. However, we show that the toxic effect of Ppz1 overexpression is unrelated to the Trk1/2 high-affinity potassium importers. Cells overexpressing Ppz1 exhibit decreased K+ content, increased cytosolic acidification, and fail to properly acidify the medium. These effects, as well as the growth defect, are counteracted by the deletion of NHA1 gene, which encodes a plasma membrane Na+, K+/H+ antiporter. The beneficial effect of a lack of Nha1 on the growth vanishes as the pH of the medium approaches neutrality, is not eliminated by the expression of two non-functional Nha1 variants (D145N or D177N), and is exacerbated by a hyperactive Nha1 version (S481A). All our results show that high levels of Ppz1 overactivate Nha1, leading to an excessive entry of H+ and efflux of K+, which is detrimental for growth.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2022
Elektronická adresa	https://www.mdpi.com/2309-608X/7/12/1010

Počet záznamů: 1