Počet záznamů: 1
Concurrent overexpression of amino acid permease AAP1 (3a) and SUT1 sucrose transporter in pea resulted in increased seed number and changed cytokinin and protein levels
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SYSNO ASEP 0549508 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Concurrent overexpression of amino acid permease AAP1 (3a) and SUT1 sucrose transporter in pea resulted in increased seed number and changed cytokinin and protein levels Tvůrce(i) Grant, J. E. (NZ)
Ninan, A. (NZ)
Cripps-Guazzone, N. (NZ)
Shaw, M. (NZ)
Song, J. (CN)
Petřík, Ivan (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Tegeder, M. (US)
Jameson, P. E. (NZ)Celkový počet autorů 9 Zdroj.dok. Functional Plant Biology. - : CSIRO Publishing - ISSN 1445-4408
Roč. 48, č. 9 (2021), s. 889-904Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova aap ; cell wall invertase ; ckx ; ipt ; legume ; Pisum sativum ; process peas ; seed coat ; seed yield ; sucrose ; sut ; sweet ; transgenic ; transgenics Obor OECD Biochemistry and molecular biology CEP GA18-07563S GA ČR - Grantová agentura ČR EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000653744600001 EID SCOPUS 85106900009 DOI 10.1071/FP21011 Anotace Using pea as our model crop, we sought to understand the regulatory control over the import of sugars and amino acids into the developing seeds and its importance for seed yield and quality. Transgenic peas simultaneously overexpressing a sucrose transporter and an amino acid transporter were developed. Pod walls, seed coats, and cotyledons were analysed separately, as well as leaves subtending developing pods. Sucrose, starch, protein, free amino acids, and endogenous cytokinins were measured during development. Temporal gene expression analyses (RT-qPCR) of amino acid (AAP), sucrose (SUT), and SWEET transporter family members, and those from cell wall invertase, cytokinin biosynthetic (IPT) and degradation (CKX) gene families indicated a strong effect of the transgenes on gene expression. In seed coats of the double transgenics, increased content and prolonged presence of cytokinin was particularly noticeable. The transgenes effectively promoted transition of young sink leaves into source leaves. We suggest the increased flux of sucrose and amino acids from source to sink, along with increased interaction between cytokinin and cell wall invertase in developing seed coats led to enhanced sink activity, resulting in higher cotyledon sucrose at process pea harvest, and increased seed number and protein content at maturity. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.1071/FP21011
Počet záznamů: 1