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Naturally zinc-containing bacteriochlorophyll a ([Zn]-BChl a) protects the photosynthetic apparatus of Acidiphilium rubrum from copper toxicity damage
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SYSNO ASEP 0548228 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Naturally zinc-containing bacteriochlorophyll a ([Zn]-BChl a) protects the photosynthetic apparatus of Acidiphilium rubrum from copper toxicity damage Tvůrce(i) Jaime-Perez, Noelia (BC-A)
Bína, David (BC-A) RID, ORCID
Kaftan, David (MBU-M) ORCID
Bokhari, Syed Nadeem Hussain (BC-A) RID
Küpper, Hendrik (BC-A) RID, ORCIDCelkový počet autorů 5 Číslo článku 148472 Zdroj.dok. Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1862, č. 10 (2021)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Bacteriochlorophyll ; Photosynthetic bacteria ; Acidiphilium rubrum ; Rhodospirillum rubrum ; Pigment-protein complexes ; Copper stress ; Reaction centre ; Trans-metallation Vědní obor RIV BO - Biofyzika Obor OECD Biophysics Vědní obor RIV – spolupráce Mikrobiologický ústav - Biochemie CEP EF15_003/0000336 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA17-14024S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 ; MBU-M - RVO:61388971 UT WOS 000681042600002 EID SCOPUS 85110290208 DOI 10.1016/j.bbabio.2021.148472 Anotace In almost all photosynthetic organisms the photosynthetic pigments chlorophyll and bacteriochlorophyll (BChl) are Mg2+ containing complexes, but Mg2+ may be exchanged against other metal ions when these are present in toxic concentrations, leading to inactivation of photosynthesis. In this report we studied mechanisms of copper toxicity to the photosynthetic apparatus of Acidiphilium rubrum, an acidophilic purple bacterium that uses Zn2+ instead of Mg2+ as the central metal in the BChl molecules ([Zn]-BChl) of its reaction centres (RCs) and light harvesting proteins (LH1). We used a combination of in vivo measurements of photosynthetic activity (fast fluorescence and absorption kinetics) together with analysis of metal binding to pigments and pigment-protein complexes by HPLC-ICP-sfMS to monitor the effect of Cu2+ on photosynthesis of A. rubrum. Further, we found that its cytoplasmic pH is neutral. We compared these results with those obtained from Rhodospirillum rubrum, a purple bacterium for which we previously reported that the central Mg2+ of BChl can be replaced in vivo in the RCs by Cu2+ under environmentally realistic Cu-2+ concentrations, leading to a strong inhibition of photosynthesis. Thus, we observed that A. rubrum is much more resistant to copper toxicity than R. rubrum. Only slight changes of photosynthetic parameters were observed in A. rubrum at copper concentrations that were severely inhibitory in R. rubrum and in A. rubrum no copper complexes of BChl were found. Altogether, the data suggest that [Zn]-BChl protects the photosynthetic apparatus of A. rubrum from detrimental insertion of Cu2+ (trans-metallation) into BChl molecules of its RCs. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0166445X21000771?via%3Dihub
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