Počet záznamů: 1
Early recognition of the PCL/fibrous carbon nanocomposites interaction with osteoblast-like cells by Raman spectroscopy
- 1.
SYSNO ASEP 0547691 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Early recognition of the PCL/fibrous carbon nanocomposites interaction with osteoblast-like cells by Raman spectroscopy Tvůrce(i) Weselucha-Birczynska, A. (PL)
Kolodziej, A. (PL)
Swietek, Malgorzata Anna (UMCH-V) RID, ORCID
Skalniak, L. (PL)
Dlugoń, E. (PL)
Pajda, M. (PL)
Blazewicz, M. (PL)Číslo článku 2890 Zdroj.dok. Nanomaterials. - : MDPI
Roč. 11, č. 11 (2021)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova nanomaterials ; poly(ε-caprolactone) (PCL) ; multi-walled carbon nanotubes (MWCNTs) Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000724858000001 EID SCOPUS 85118109422 DOI 10.3390/nano11112890 Anotace Poly(ε-caprolactone) (PCL) is a biocompatible resorbable material, but its use is limited due to the fact that it is characterized by the lack of cell adhesion to its surface. Various chemical and physical methods are described in the literature, as well as modifications with various nanoparticles aimed at giving it such surface properties that would positively affect cell adhesion. Nanomaterials, in the form of membranes, were obtained by the introduction of multi-walled carbon nanotubes (MWCNTs and functionalized nanotubes, MWCNTs-f) as well as electro-spun carbon nanofibers (ESCNFs, and functionalized nanofibers, ESCNFs-f) into a PCL matrix. Their properties were compared with that of reference, unmodified PCL membrane. Human osteoblast-like cell line, U-2 OS (expressing green fluorescent protein, GFP) was seeded on the evaluated nanomaterial membranes at relatively low confluency and cultured in the standard cell culture conditions. The attachment and the growth of the cell populations on the polymer and nanocomposite samples were monitored throughout the first week of culture with fluorescence microscopy. Simultaneously, Raman microspectroscopy was also used to track the dependence of U-2 OS cell development on the type of nanomaterial, and it has proven to be the best method for the early detection of nanomaterial/cell interactions. The differentiation of interactions depending on the type of nanoadditive is indicated by the ν(COC) vibration range, which indicates the interaction with PCL membranes with carbon nanotubes, while it is irrelevant for PCL with carbon nanofibers, for which no changes are observed. The vibration range ω(CH2) indicates the interaction for PCL with carbon nanofibers with seeded cells. The crystallinity of the area ν(C=O) increases for PCL/MWCNTs and for PCL/MWCNTs-f, while it decreases for PCL/ESCNFs and for PCL/ESCNFs-f with seeded cells. The crystallinity of the membranes, which is determined by Raman microspectroscopy, allows for the assessment of polymer structure changes and their degradability caused by the secretion of cell products into the ECM and the differentiation of interactions depending on the carbon nanostructure. The obtained nanocomposite membranes are promising bioactive materials. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2079-4991/11/11/2890
Počet záznamů: 1