Počet záznamů: 1
TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley
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SYSNO ASEP 0547377 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název TALEN-Based HvMPK3 Knock-Out Attenuates Proteome and Root Hair Phenotypic Responses to flg22 in Barley Tvůrce(i) Takáč, T. (CZ)
Křenek, P. (CZ)
Komis, G. (CZ)
Vadovič, P. (CZ)
Ovečka, M. (CZ)
Ohnoutková, Ludmila (UEB-Q) RID, ORCID
Pechan, T. (US)
Kašpárek, Petr (UMG-J)
Tichá, T. (CZ)
Basheer, J. (CZ)
Arick, M. (US)
Šamaj, J. (CZ)Celkový počet autorů 12 Číslo článku 666229 Zdroj.dok. Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 12, APR 29 (2021)Poč.str. 23 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova barley ; chitinases ; flagellin ; HvMPK3 ; PR proteins ; proteomics ; root hairs ; talen Obor OECD Biochemistry and molecular biology CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 ; UMG-J - RVO:68378050 UT WOS 000649834600001 EID SCOPUS 85105936605 DOI 10.3389/fpls.2021.666229 Anotace Mitogen activated protein kinases (MAPKs) integrate elicitor perception with both early and late responses associated with plant defense and innate immunity. Much of the existing knowledge on the role of plant MAPKs in defense mechanisms against microbes stems from extensive research in the model plant Arabidopsis thaliana. In the present study, we investigated the involvement of barley (Hordeum vulgare) MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. Using differential proteomic analysis we show that TALEN-induced MPK3 knock-out lines of barley (HvMPK3 KO) exhibit constitutive downregulation of defense related proteins such as PR proteins belonging to thaumatin family and chitinases. Further analyses showed that the same protein families were less prone to flg22 elicitation in HvMPK3 KO plants compared to wild types. These results were supported and validated by chitinase activity analyses and immunoblotting for HSP70. In addition, differential proteomes correlated with root hair phenotypes and suggested tolerance of HvMPK3 KO lines to flg22. In conclusion, our study points to the specific role of HvMPK3 in molecular and root hair phenotypic responses of barley to flg22. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.3389/fpls.2021.666229
Počet záznamů: 1