Počet záznamů: 1

Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect

SYSNO ASEP	0546834
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
Tvůrce(i)	Bárcenas-Pérez, Daniela (MBU-M)_{ORCID, RID} Střížek, Antonín (MBU-M) Hrouzek, Pavel (MBU-M)_ORCID Kopecký, Jan (MBU-M) Barradas, M. (ES) Sierra-Ramirez, A. (ES) Fernandez-Marcos, P. J. (ES) Cheel, José (MBU-M)_{RID, ORCID}
Číslo článku	517
Zdroj.dok.	Marine Drugs. - : MDPI Roč. 19, č. 9 (2021)
Poč.str.	19 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	centrifugal partition chromatography ; undaria-pinnatifida ; fucoxanthin ; Phaeodactylum tricornutum ; high performance countercurrent chromatography (HPCCC) ; countercurrent chromatography (CCC) ; centrifugal partition chromatography (CPC)
Vědní obor RIV	EE - Mikrobiologie, virologie
Obor OECD	Microbiology
CEP	TN01000048 GA TA ČR - Technologická agentura ČR
	LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000699484700001
EID SCOPUS	85116599987
DOI	10.3390/md19090517
Anotace	Phaeodactylum tricornutum is a rich source of fucoxanthin, a carotenoid with several health benefits. In the present study, high performance countercurrent chromatography (HPCCC) was used to isolate fucoxanthin from an extract of P. tricornutum. A multiple sequential injection HPCCC method was developed combining two elution modes (reverse phase and extrusion). The lower phase of a biphasic solvent system (n-heptane, ethyl acetate, ethanol and water, ratio 5/5/6/3, v/v/v/v) was used as the mobile phase, while the upper phase was the stationary phase. Ten consecutive sample injections (240 mg of extract each) were performed leading to the separation of 38 mg fucoxanthin with purity of 97% and a recovery of 98%. The process throughput was 0.189 g/h, while the efficiency per gram of fucoxanthin was 0.003 g/h. Environmental risk and general process evaluation factors were used for assessment of the developed separation method and compared with existing fucoxanthin liquid-liquid isolation methods. The isolated fucoxanthin retained its well-described ability to induce nuclear translocation of transcription factor FOXO3. Overall, the developed isolation method may represent a useful model to produce biologically active fucoxanthin from diatom biomass.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2022
Elektronická adresa	https://www.mdpi.com/1660-3397/19/9/517

Počet záznamů: 1