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Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect
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SYSNO ASEP 0546834 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Production of Fucoxanthin from Phaeodactylum tricornutum Using High Performance Countercurrent Chromatography Retaining Its FOXO3 Nuclear Translocation-Inducing Effect Tvůrce(i) Bárcenas-Pérez, Daniela (MBU-M) ORCID, RID
Střížek, Antonín (MBU-M)
Hrouzek, Pavel (MBU-M) ORCID
Kopecký, Jan (MBU-M)
Barradas, M. (ES)
Sierra-Ramirez, A. (ES)
Fernandez-Marcos, P. J. (ES)
Cheel, José (MBU-M) RID, ORCIDČíslo článku 517 Zdroj.dok. Marine Drugs. - : MDPI
Roč. 19, č. 9 (2021)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova centrifugal partition chromatography ; undaria-pinnatifida ; fucoxanthin ; Phaeodactylum tricornutum ; high performance countercurrent chromatography (HPCCC) ; countercurrent chromatography (CCC) ; centrifugal partition chromatography (CPC) Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP TN01000048 GA TA ČR - Technologická agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000699484700001 EID SCOPUS 85116599987 DOI 10.3390/md19090517 Anotace Phaeodactylum tricornutum is a rich source of fucoxanthin, a carotenoid with several health benefits. In the present study, high performance countercurrent chromatography (HPCCC) was used to isolate fucoxanthin from an extract of P. tricornutum. A multiple sequential injection HPCCC method was developed combining two elution modes (reverse phase and extrusion). The lower phase of a biphasic solvent system (n-heptane, ethyl acetate, ethanol and water, ratio 5/5/6/3, v/v/v/v) was used as the mobile phase, while the upper phase was the stationary phase. Ten consecutive sample injections (240 mg of extract each) were performed leading to the separation of 38 mg fucoxanthin with purity of 97% and a recovery of 98%. The process throughput was 0.189 g/h, while the efficiency per gram of fucoxanthin was 0.003 g/h. Environmental risk and general process evaluation factors were used for assessment of the developed separation method and compared with existing fucoxanthin liquid-liquid isolation methods. The isolated fucoxanthin retained its well-described ability to induce nuclear translocation of transcription factor FOXO3. Overall, the developed isolation method may represent a useful model to produce biologically active fucoxanthin from diatom biomass. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1660-3397/19/9/517
Počet záznamů: 1