Počet záznamů: 1
Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica
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SYSNO ASEP 0546812 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica Tvůrce(i) Miláček, Matěj (BC-A) ORCID
Bittová, Lenka (BC-A) RID, ORCID
Tůmová, Šárka (BC-A) ORCID
Lukšan, Ondřej (UOCHB-X)
Hanus, Robert (UOCHB-X) RID, ORCID
Kyjaková, Pavlína (UOCHB-X) RID
Machara, Aleš (UOCHB-X) ORCID
Marek, Aleš (UOCHB-X) RID, ORCID
Jindra, Marek (BC-A) RID, ORCIDCelkový počet autorů 9 Číslo článku 103671 Zdroj.dok. Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748
Roč. 139, DEC 01 (2021)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova juvenile hormone ; methoprene-tolerant ; hormone receptor Vědní obor RIV ED - Fyziologie Obor OECD Biochemistry and molecular biology CEP GX20-05151X GA ČR - Grantová agentura ČR LM2015064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 ; UOCHB-X - RVO:61388963 UT WOS 000711780700006 EID SCOPUS 85117203283 DOI 10.1016/j.ibmb.2021.103671 Anotace Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai). Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism. Studies addressing the agonist ligand binding to recombinant JH receptors currently include three species belonging to two holometabolous insect orders, but none that would represent any of the hemimetabolous orders. Here, we examined JH receptors in two representatives of Blattodea, the cockroach Blattella germanica and the termite Prorhinotermes simplex. To test the JH-binding capacity of Met proteins from these species, we performed chemical synthesis and tritium labeling of the natural blattodean JH homolog, JH III. Our improved protocol increased the yield and specific activity of [10-3H]JH III relative to formerly available preparations. Met proteins from both species specifically bound [3H]JH III with high affinity, whereas Met variants mutated at a critical position within the ligand-binding domain were incapable of such binding. Furthermore, JH III and the synthetic JH mimic fenoxycarb stimulated dimerization between Met and Tai components of the respective JH receptors of both species. These data present primary evidence for agonist binding by JH receptors in any hemimetabolous species and provide a molecular basis for JH action in cockroaches and termites. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0965174821001545?via%3Dihub
Počet záznamů: 1