Počet záznamů: 1
Cyanochelins, an Overlooked Class of Widely Distributed Cyanobacterial Siderophores, Discovered by Silent Gene Cluster Awakening
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SYSNO ASEP 0546127 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cyanochelins, an Overlooked Class of Widely Distributed Cyanobacterial Siderophores, Discovered by Silent Gene Cluster Awakening Tvůrce(i) Galica, Tomáš (MBU-M) RID
Borbone, N. (IT)
Mareš, Jan (MBU-M) ORCID
Kust, Andreja (MBU-M) ORCID
Caso, A. (IT)
Esposito, G. (IT)
Saurav, Kumar (MBU-M) ORCID
Hájek, Jan (MBU-M) ORCID
Řeháková, Klára (BU-J) RID, ORCID
Urajová, Petra (MBU-M)
Costantino, V. (IT)
Hrouzek, Pavel (MBU-M) ORCIDČíslo článku e03128-20 Zdroj.dok. Applied and Environmental Microbiology. - : American Society for Microbiology - ISSN 0099-2240
Roč. 87, č. 17 (2021)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cyanobacteria ; iron acquisition ; lipopeptides ; secondary metabolism ; siderophores Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA16-09381S GA ČR - Grantová agentura ČR GJ19-17868Y GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 ; BU-J - RVO:67985939 UT WOS 000693759300028 EID SCOPUS 85114119775 DOI 10.1128/AEM.03128-20 Anotace Cyanobacteria require iron for growth and often inhabit iron-limited habitats, yet only a few siderophores are known to be produced by them. We report that cyanobacterial genomes frequently encode polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) biosynthetic pathways for synthesis of lipopeptides featuring beta-hydroxyaspartate (beta-OH-Asp), a residue known to be involved in iron chelation. Iron starvation triggered the synthesis of beta-OH-Asp lipopeptides in the cyanobacteria Rivularia sp. strain PCC 7116, Leptolyngbya sp. strain NIES-3755, and Rubidibacter lacunae strain KORDI 51-2. The induced compounds were confirmed to bind iron by mass spectrometry (MS) and were capable of Fe3+ to Fe2+ photoreduction, accompanied by their cleavage, when exposed to sunlight. The siderophore from Rivularia, named cyanochelin A, was structurally characterized by MS and nuclear magnetic resonance (NMR) and found to contain a hydrophobic tail bound to phenolate and oxazole moieties followed by five amino acids, including two modified aspartate residues for iron chelation. Phylogenomic analysis revealed 26 additional cyanochelin-like gene clusters across a broad range of cyanobacterial lineages. Our data suggest that cyanochelins and related compounds are widespread beta-OH-Asp-featuring cyanobacterial siderophores produced by phylogenetically distant species upon iron starvation. Production of photolabile siderophores by phototrophic cyanobacteria raises questions about whether the compounds facilitate iron monopolization by the producer or, rather, provide Fe2+ for the whole microbial community via photoreduction. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://journals.asm.org/doi/epub/10.1128/AEM.03128-20
Počet záznamů: 1