Počet záznamů: 1
Characterization of alba family expression and localization in arabidopsis thaliana generative organs
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SYSNO ASEP 0545878 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of alba family expression and localization in arabidopsis thaliana generative organs Tvůrce(i) Náprstková, Alena (UEB-Q) ORCID
Malínská, Kateřina (UEB-Q) RID, ORCID
Záveská Drábková, Lenka (UEB-Q) ORCID
Billey, E. (FR)
Náprstková, Dagmar (UEB-Q)
Sýkorová, Eva (BFU-R) RID, ORCID
Bousquet-Antonelli, C. (FR)
Honys, David (UEB-Q) RID, ORCIDCelkový počet autorů 8 Číslo článku 1652 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 4 (2021)Poč.str. 23 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova alba ; Arabidopsis thaliana ; Confocal microscopy ; Expression analysis ; Flowering ; Heat stress ; pabp3 ; Pollen development ; Protein localization Obor OECD Plant sciences, botany CEP GA18-07027S GA ČR - Grantová agentura ČR EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 ; BFU-R - RVO:68081707 UT WOS 000623761000001 EID SCOPUS 85100487109 DOI 10.3390/ijms22041652 Anotace ALBA DNA/RNA-binding proteins form an ancient family, which in eukaryotes diversi-fied into two Rpp25-like and Rpp20-like subfamilies. In most studied model organisms, their function remains unclear, but they are usually associated with RNA metabolism, mRNA translatability and stress response. In plants, the enriched number of ALBA family members remains poorly understood. Here, we studied ALBA dynamics during reproductive development in Ara-bidopsis at the levels of gene expression and protein localization, both under standard conditions and following heat stress. In generative tissues, ALBA proteins showed the strongest signal in mature pollen where they localized predominantly in cytoplasmic foci, particularly in regions surrounding the vegetative nucleus and sperm cells. Finally, we demonstrated the involvement of two Rpp25-like subfamily members ALBA4 and ALBA6 in RNA metabolism in mature pollen supported by their co-localization with poly(A)-binding protein 3 (PABP3). Collectively, we demonstrated the engagement of ALBA proteins in male reproductive development and the heat stress response, highlighting the involvement of ALBA4 and ALBA6 in RNA metabolism, storage and/or translational control in pollen upon heat stress. Such dynamic re-localization of ALBA proteins in a controlled, developmentally and environmentally regulated manner, likely reflects not only their redundancy but also their possible functional diversification in plants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.3390/ijms22041652
Počet záznamů: 1