Počet záznamů: 1
Thermoresponsive properties of polyacrylamides in physiological solutions
- 1.
SYSNO ASEP 0545564 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Thermoresponsive properties of polyacrylamides in physiological solutions Tvůrce(i) Kolouchová, Kristýna (UMCH-V) ORCID, RID
Lobaz, Volodymyr (UMCH-V) RID, ORCID
Beneš, Hynek (UMCH-V) RID, ORCID
de la Rosa, V. R. (BE)
Babuka, David (UMCH-V)
Švec, Pavel (UMCH-V) RID, ORCID
Černoch, Peter (UMCH-V) RID, ORCID
Hrubý, Martin (UMCH-V) RID, ORCID
Hoogenboom, R. (BE)
Štěpánek, Petr (UMCH-V) RID, ORCID
Groborz, Ondřej (UMCH-V) ORCID, RIDZdroj.dok. Polymer Chemistry . - : Royal Society of Chemistry - ISSN 1759-9954
Roč. 12, č. 35 (2021), s. 5077-5084Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova lower critical solution temperature ; LCST ; cloud point temperature Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GA19-01602S GA ČR - Grantová agentura ČR TO01000074 GA TA ČR - Technologická agentura ČR Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000687745000001 EID SCOPUS 85115181597 DOI 10.1039/D1PY00843A Anotace Polymer solutions with a lower critical solution temperature (LCST) undergo reversible phase separation when heated above their cloud point temperature (TCP or CPT). As such, they have been proposed for a wide range of biomedical applications, from injectable drug depots to switchable coatings for cell adhesion. However, in systematic studies, the TCP of these thermoresponsive polymers has been mostly measured in non-physiological solutions, thereby hindering the development of their medicinal applications. Here, we analysed the thermoresponsive properties of four acrylamide-based polymers with LCST, namely poly[(N-2,2-difluoroethyl)acrylamide] (pDFEA), poly[(N-isopropyl)acrylamide] (pNIPAM), poly[(N,N-diethyl)acrylamide] (pDEA), and poly[(N-acryloyl)pyrrolidine] (pAP). As shown by turbidimetry, their TCP in phosphate saline buffer (PBS) and foetal bovine serum (FBS) were consistently lower than those reported in the literature, typically assessed in pure water, even when using the same setup. In addition, these physiological solutions affected the variation of TCP as a function of polymer concentration (1.25 to 10.0 mg mL−1) and molar mass (20 to 50 kg mol−1). As shown by isothermal calorimetry, interactions between proteins in FBS and polymer aggregates were predominantly exothermic, which indicates that protein–polymer complexes are formed through enthalpically driven processes. In conclusion, the TCP of thermoresponsive polymers strongly depends on solvent composition and therefore should be measured under physiological conditions for future medicinal applications.
Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2022 Elektronická adresa https://pubs.rsc.org/en/content/articlelanding/2021/PY/D1PY00843A
Počet záznamů: 1