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Transcriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression
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SYSNO ASEP 0544993 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transcriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression Tvůrce(i) Mangiola, S. (AU)
McCoy, P. (AU)
Modrák, Martin (MBU-M) ORCID
Souza-Fonseca-Guimaraes, F. (AU)
Blashki, D. (AU)
Stuchbery, R. (AU)
Keam, S. P. (AU)
Kerger, M. (AU)
Chow, K. (AU)
Nasa, C. (AU)
Le Page, M. (AU)
Lister, N. (AU)
Monard, S. (AU)
Peters, J. (AU)
Dundee, P. (AU)
Williams, S. G. (AU)
Costello, A. J. (AU)
Neeson, P. J. (AU)
Pal, B. (AU)
Huntington, N. D. (AU)
Corcoran, N. M. (AU)
Papenfuss, A. T. (AU)
Hovens, C. M. (AU)Číslo článku 846 Zdroj.dok. Bmc Cancer. - : BioMed Central
Roč. 21, č. 1 (2021)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Prostate cancer ; Transcriptomics ; facs ; Immunohistochemistry ; Deconvolution ; Bayes ; Differential gene expression ; capra-s ; Microenvironment ; Epithelial ; Myeloid ; Macrophages ; Cholesterol ; pdl1 Vědní obor RIV FD - Onkologie a hematologie Obor OECD Oncology CEP LM2015047 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000680321500003 EID SCOPUS 85111046075 DOI 10.1186/s12885-021-08529-6 Anotace Background Prostate cancer is caused by genomic aberrations in normal epithelial cells, however clinical translation of findings from analyses of cancer cells alone has been very limited. A deeper understanding of the tumour microenvironment is needed to identify the key drivers of disease progression and reveal novel therapeutic opportunities. Results In this study, the experimental enrichment of selected cell-types, the development of a Bayesian inference model for continuous differential transcript abundance, and multiplex immunohistochemistry permitted us to define the transcriptional landscape of the prostate cancer microenvironment along the disease progression axis. An important role of monocytes and macrophages in prostate cancer progression and disease recurrence was uncovered, supported by both transcriptional landscape findings and by differential tissue composition analyses. These findings were corroborated and validated by spatial analyses at the single-cell level using multiplex immunohistochemistry. Conclusions This study advances our knowledge concerning the role of monocyte-derived recruitment in primary prostate cancer, and supports their key role in disease progression, patient survival and prostate microenvironment immune modulation. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://bmccancer.biomedcentral.com/articles/10.1186/s12885-021-08529-6
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