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Recommendations on the quantitative analysis of pheophorbides, photosensitizers present in algal biomass intended as food supplement

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    SYSNO ASEP0544796
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevRecommendations on the quantitative analysis of pheophorbides, photosensitizers present in algal biomass intended as food supplement
    Tvůrce(i) Činčárová, Dominika (MBU-M)
    Hájek, Jan (MBU-M) ORCID
    Dobřichovský, M. (CZ)
    Lukeš, Martin (MBU-M) ORCID
    Hrouzek, Pavel (MBU-M) ORCID
    Celkový počet autorů5
    Číslo článku102298
    Zdroj.dok.Algal Research-Biomass Biofuels and Bioproducts - ISSN 2211-9264
    Roč. 56, JUNE 2021 (2021)
    Poč.str.10 s.
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaPheophorbide a ; Analysis ; Extraction ; Chlorophyllase inactivation ; Algae ; Chlorophyll degradation
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPTN01000048 GA TA ČR - Technologická agentura České republiky
    Způsob publikováníOmezený přístup
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000663410300004
    EID SCOPUS85105254433
    DOI10.1016/j.algal.2021.102298
    AnotacePheophorbide a is the major photosensitizer present in algal biomass and its presence in food supplement was linked to human health issues in the past. Although several pheophorbide a quantification methods have been proposed including the United States Pharmacopeia-National Formulary protocol (USP-NFP), there are open methodological issues which make their results questionable. In the present study we have tested optimal extraction conditions for pheophorbide a from a disintegrated biomass of green algae Chlorella and Haematococcus, widely used as food supplement. Further we have compared the quantification of pheophorbide a in algal extracts using HPLC-HRMS with the spectrophotometric quantification after USP-NFP in Haematococcus biomass and oleoresin samples. We have demonstrated that USP-NFP provide considerably (1.7-2.4 times) higher pheophorbide contents compared to HPLC-HRMS quantification using a pheophorbide a analytical standard. This was mainly caused by the fact that USP-NFP integrates the concentration of all pheophorbide variants present in the samples. We have detected nine pheophorbide analogues in the original algal sample as well as in the final sample of USP-NFP, of which pheophorbide a represented only similar to 40%. However, this explains the values obtained by USP-NFP only partly. We also show that 60% acetone is the most suitable extraction solvent for pheophorbide a from various algal samples, however, we noted that such extracts might exhibit high activity of chlorophyllase generating pheophorbide a during the extraction process. This activity differed between studied Chlorella and Haematococcus biomass extracts. We have achieved the chlorophyllase inactivation by a short-term high-temperature treatment (90 degrees C, 5 min). Acceptable stability of pheophorbide a and only minor degradation of chlorophyll to pheophorbide a has been observed at these conditions.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2022
    Elektronická adresahttps://www.sciencedirect.com/science/article/pii/S221192642100117X?via%3Dihub
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