Počet záznamů: 1
The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata)
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SYSNO ASEP 0544658 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata) Tvůrce(i) Abeyawardana, Oushadee A.J. (UEB-Q) ORCID
Viehmannová, I. (CZ)
Koudela, M. (CZ)Celkový počet autorů 3 Zdroj.dok. Journal of Horticultural Research. - : Instytut Ogrodnictwa - ISSN 2300-5009
Roč. 29, č. 1 (2021), s. 47-54Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova cabbage ; explants ; in vitro culture ; polyploids ; regeneration ; trifluralin Obor OECD Agricultural biotechnology and food biotechnology Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 EID SCOPUS 85106380134 DOI 10.2478/johr-2021-0007 Anotace Six genotypes of head cabbage ('Flexima', 'Mutsuma', 'Septima', 'Zeus', DC6, 'Ancoma') have been studied to find an effective method of obtaining polyploids using trifluralin. The effect of various trifluralin concentrations (0.5, 1.0, 1.5, and 2.0 mg·L-1) and treatment conditions (24 °C/24 h, 24 °C/48 h, 30 °C/24 h, and 30 °C/48 h) on organogenesis of organogenic callus, hypocotyl, and seeds was tested. Ploidy level was detected using a flow cytometer. As a result, low survival of hypocotyls and calli was obtained. The shoots induced from calli and hypocotyls failed to develop quality plantlets and most calli and hypocotyls remained recalcitrant for further differentiation. Treated seeds seemed to be promising objects basing on a considerable percentage of plant regeneration. With the increased concentration of trifluralin and increasing press of treatment conditions, the seed germination rate was significantly reduced. Shoot induction from germinated seeds varied among genotypes and treatments. The root induction was independent of treatments but also reduced considerably compared to the control. Under tested conditions, none of the polyploids resulted. Further studies are required to be successful in protocol development. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.2478/johr-2021-0007
Počet záznamů: 1