Počet záznamů: 1
Monitoring fitness and productivity in cyanobacteria batch cultures
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SYSNO ASEP 0543490 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Monitoring fitness and productivity in cyanobacteria batch cultures Tvůrce(i) Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Schoffman, H. (IL)
Lukeš, Martin (MBU-M) ORCID
Fedorko, Jan (UEK-B) SAI, ORCID
Keren, N. (IL)
Červený, Jan (UEK-B) RID, ORCID, SAICelkový počet autorů 6 Číslo článku 102328 Zdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 56, JUN (2021)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova sp pcc 6803 ; photosystem-ii ; microalgae cultivation ; dark respiration ; growth-rate ; light ; phytoplankton ; photosynthesis ; fluorescence ; algae ; Batch cultivation ; Cyanobacteria ; Excitation-emission spectra ; Fitness ; Metal uptake ; Photosynthesis Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Mikrobiologický ústav - Mikrobiologie, virologie CEP LO1415 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy GA18-24397S GA ČR - Grantová agentura ČR EF16_026/0008413 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy TN01000048 GA TA ČR - Technologická agentura České republiky Výzkumná infrastruktura CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Způsob publikování Omezený přístup Institucionální podpora UEK-B - RVO:86652079 ; MBU-M - RVO:61388971 UT WOS 000654306500008 EID SCOPUS 85108537722 DOI 10.1016/j.algal.2021.102328 Anotace Cyanobacteria are key photosynthetic organisms in many aquatic ecosystems and hold great potential for sustainable green biotechnology. Growth of cyanobacteria in batch cultures is expected to be part of future biotechnological practices. However, the issue of correlating the dynamics of metabolic and photosynthetic parameters with the culture fitness during batch cultivation is still outstanding. In this paper we take advantage of a photobioreactor system to continuously track growth parameters of Synechocystis sp. PCC 6803, and to couple online culture monitoring with offline measurements of photosynthetic efficiency and biochemical and elemental cell composition under several light intensity and CO2 regimes. Light intensity determines the flux of energy into the photosynthetic system while CO2 concentrations determines the ability to capture this energy in chemical form. From this perspective, four distinct source-sink regimes were established and compared, which allowed us to reveal specific strategies to acclimate to both carbon and light limitation. As part of the measurements, room temperature excitation-emission spectra and elemental composition of Synechocystis cells were, for the first time, compared throughout the exponential and linear growth phases. In total, 39 parameters (out of 170 measured) were identified as highly correlating (R2 > 0.9) with growth rate or productivity under at least one tested cultivation condition, including concentrations and ratios of pigments or particular elements. For online fitness and productivity monitoring in cyanobacteria batch cultures, parameters such as photosynthesis and respiration rates and ratios, energy-dependent non-photochemical quenching (qE) or Zn and Mo concentration in the cultivation medium can be of interest. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, audesova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2211926421001478?via%3Dihub
Počet záznamů: 1