Počet záznamů: 1
Microfluidic on-chip microwave sensing of the self-assembly state of tubulin
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SYSNO ASEP 0543281 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Microfluidic on-chip microwave sensing of the self-assembly state of tubulin Tvůrce(i) Krivosudský, Ondrej (URE-Y)
Havelka, Daniel (URE-Y) RID
Chafai, Djamel Eddine (URE-Y)
Cifra, Michal (URE-Y) RID, ORCID, SAICelkový počet autorů 4 Číslo článku 129068 Zdroj.dok. Sensors and Actuators B - Chemical. - : Elsevier
Roč. 328, FEB 1 2021 (2021)Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Self-assembly ; Proteins ; Tubulin ; Radiofrequency and microwave chips ; High-frequency biosensors Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika Obor OECD Electrical and electronic engineering CEP GX20-06873X GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora URE-Y - RVO:67985882 UT WOS 000600962800008 DOI 10.1016/j.snb.2020.129068 Anotace Self-assembly is at the heart of many promising nanoscience technologies as well as at the core of life processes. Tubulin proteins self-assemble into microtubules, tube-like structures that are essential in cellular functions such as cell division and intracellular transport and also a major target in cancer therapies. Therefore, it is crucial to develop efficient methods for monitoring tubulin self-assembly at the molecular level. To mitigate the limitations of current methods, we propose a new approach based on a microwave lab-on-a-chip method to monitor tubulin self-assembly states. To that end, we designed a dedicated microwave platform with integrated microfluidics with a sensing volume of < 30 nL. In parallel, we used a standard bulk light-scattering-based method to assess the tubulin self-assembly and to validate the microwave chip results. Using our chip, for the first time, we demonstrate that the self-assembly state of tubulin into microtubules can be monitored using microwave microfluidics technology. Our results introduce a novel label-free electromagnetic monitoring and analytical method for bionanotechnology and biomedicine applications that can potentially be integrated into advanced microscopy systems Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2022 Elektronická adresa https://doi.org/10.1016/j.snb.2020.129068
Počet záznamů: 1