Počet záznamů: 1
Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa
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SYSNO ASEP 0542929 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa Tvůrce(i) Čaja, Fabian (MBU-M) ORCID
Stakheev, Dmitry (MBU-M)
Chernyavskiy, Oleksandr (FGU-C)
Kubínová, Lucie (FGU-C) RID, ORCID
Křižan, Jiří (MBU-M) RID
Dvořák, Jiří (MBU-M) RID, ORCID
Rossmann, Pavel (MBU-M)
Štěpánková, Renata (MBU-M) RID
Makovicky, Pe. (SK)
Makovicky, Pa. (SK)
Vymetálková, Veronika (UEM-P) RID
Souček, P. (CZ)
Vodička, Pavel (UEM-P) RID
Vodičková, Ludmila (UEM-P) RID
Levý, M. (CZ)
Vannucci, Luca (MBU-M) RID, ORCIDČíslo článku 2463 Zdroj.dok. Cancers (Basel). - : MDPI
Roč. 13, č. 10 (2021)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova colorectal cancer ; DSS-induced colitis ; chronic inflammation ; collagen ; tissue scaffold ; aom ; tumour niche ; il-6 Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Fyziologický ústav - Morfologické obory a cytologie
Ústav experimentální medicínyCEP IAA500200917 GA AV ČR - Akademie věd Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; FGU-C - RVO:67985823 ; UEM-P - RVO:68378041 UT WOS 000654722200001 EID SCOPUS 85105863368 DOI 10.3390/cancers13102463 Anotace Chronic colitis and colon cancer develop for alteration of the mucosa homeostatic regulation, also involving TGF-beta 1. Dextran sulphate sodium (DSS)-induced colitis and azoxymethane (AOM)-induced colorectal carcinogenesis animal models allow for the investigation of the pathological evolution steps. Since chronic inflammation is a common factor, we aimed to explore in rat models the colon mucosa immunological and structural conditions at one month after the end of the inductions, a transition period between acute effects and established lesions. We found, in comparison to healthy controls, downregulation of inflammatory cytokines (except IL-6) and of TGF-beta 1. At the same time, the collagen scaffold was significantly remodelled in both groups. We conclude that the pro-inflammatory cytokines, in front of a downregulated TGF-beta 1, sustained a smouldering inflammation with structural changes preparing the niche of both pathologies (ulcerative colitis with fibrosis, tumour). The collagen scaffold changes pointing to an unnoticed inflammation may be suggested as a possible pre-neoplastic condition marker.
Continuous activation of the immune system inside a tissue can lead to remodelling of the tissue structure and creation of a specific microenvironment, such as during the tumour development. Chronic inflammation is a central player in stimulating changes that alter the tissue stroma and can lead to fibrotic evolution. In the colon mucosa, regulatory mechanisms, including TGF-beta 1, avoid damaging inflammation in front of the continuous challenge by the intestinal microbiome. Inducing either DSS colitis or AOM colorectal carcinogenesis in AVN-Wistar rats, we evaluated at one month after the end of each treatment whether immunological changes and remodelling of the collagen scaffold were already in development. At this time point, we found in both models a general downregulation of pro-inflammatory cytokines and even of TGF-beta 1, but not of IL-6.Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2072-6694/13/10/2463
Počet záznamů: 1