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Degradation Products of Polychlorinated Biphenyls and Their In Vitro Transformation by Ligninolytic Fungi
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SYSNO ASEP 0542795 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Degradation Products of Polychlorinated Biphenyls and Their In Vitro Transformation by Ligninolytic Fungi Tvůrce(i) Šrédlová, Kamila (MBU-M) ORCID
Šírová, Kateřina (MBU-M)
Stella, Tatiana (MBU-M)
Cajthaml, Tomáš (MBU-M) RID, ORCIDČíslo článku 81 Zdroj.dok. Toxics. - : MDPI
Roč. 9, č. 4 (2021)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova hydroxylated PCBs ; chlorobenzyl alcohols ; chlorobenzaldehydes ; biodegradation ; Pleurotus ostreatus ; Irpex lacteus Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Environmental sciences (social aspects to be 5.7) Vědní obor RIV – spolupráce Mikrobiologický ústav - Znečištění a kontrola vody Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000643628200001 EID SCOPUS 85104856938 DOI 10.3390/toxics9040081 Anotace Metabolites of polychlorinated biphenyls (PCBs)-hydroxylated PCBs (OH-PCBs), chlorobenzyl alcohols (CB-OHs), and chlorobenzaldehydes (CB-CHOs)-were incubated in vitro with the extracellular liquid of Pleurotus ostreatus, which contains mainly laccase and low manganese-dependent peroxidase (MnP) activity. The enzymes were able to decrease the amount of most of the tested OH-PCBs by > 80% within 1 h, the removal of more recalcitrant OH-PCBs was greatly enhanced by the addition of the laccase mediator syringaldehyde. Conversely, glutathione substantially hindered the reaction, suggesting that it acted as a laccase inhibitor. Hydroxylated dibenzofuran and chlorobenzoic acid were identified as transformation products of OH-PCBs. The extracellular enzymes also oxidized the CB-OHs to the corresponding CB-CHOs on the order of hours to days, however, the mediated and nonmediated setups exhibited only slight differences, and the participating enzymes could not be determined. When CB-CHOs were used as the substrates, only partial transformation was observed. In an additional experiment, the extracellular liquid of Irpex lacteus, which contains predominantly MnP, was able to efficiently transform CB-CHOs with the aid of glutathione, mono- and di-chloroacetophenones were detected as transformation products. These results demonstrate that extracellular enzymes of ligninolytic fungi can act on a wide range of PCB metabolites, emphasizing their potential for bioremediation. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2305-6304/9/4/81
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