Počet záznamů: 1
A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography
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SYSNO ASEP 0542764 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography Tvůrce(i) Bárcenas-Pérez, Daniela (MBU-M) ORCID, RID
Lukeš, Martin (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Kubáč, David (MBU-M) RID, ORCID
Kopecký, Jiří (MBU-M) ORCID
Kaštánek, P. (CZ)
Cheel, José (MBU-M) RID, ORCIDČíslo článku 102241 Zdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 55, MAY 2021 (2021)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Docosahexaenoic acid (DHA) ; Docosapentaenoic acid n-6 (DPA n-6) ; Schizochytrium limacinum ; Countercurrent chromatography (CCC) ; High performance countercurrent ; chromatography (HPCCC) ; Centrifugal partition chromatography (CPC) Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP TN01000048 GA TA ČR - Technologická agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000642452900004 EID SCOPUS 85101814625 DOI 10.1016/j.algal.2021.102241 Anotace Schizochytrium has arisen as a valuable alternative to fish as a source of polyunsaturated fatty acids. In the present study, Schizochytrium limacinum CO3-H biomass was processed by direct transesterification affording a transesterified algal oil, from which docosahexaenoic acid (DHA) and docosapentaenoic acid n-6 (DPA n-6) ethyl esters were isolated using high performance countercurrent chromatography (HPCCC). A multiple sequential injection HPCCC separation method was developed combining two elution modes (reverse phase and extrusion). During the reverse phase elution, the lower phase of a biphasic solvent system (n-heptane:acetonitrile, ratio 5:5, v/v) was used as the mobile phase. During the initial reverse phase elution, the two target compounds were obtained, which was followed by the extrusion of the stationary phase by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation. Once the column was refilled with stationary phase, a new hydrodynamic equilibrium condition was again reached for a new separation cycle. Ten consecutive sample injections (1000 mg of algal oil, each) were performed in this way leading to the separation of DHA ethyl ester (1, 797 mg, 99% purity) and DPA n-6 ethyl ester (2, 164 mg, 97% purity) with recoveries of 99% and 92%, respectively. The process throughput of algal oil was 1.149 g/h while the efficiency per gram of two target compounds was 0.110 g/h. Environmental risk and process evaluation factors were used for evaluation of the separation process. Overall, this separation strategy may represent a useful model for the coproduction of DHA and DPA n-6 from microalgae oil under a biorefinery perspective because the potential coproduction of other valuable compounds present in both the algal oil and biomass may contribute to enhancement of the economics of the approach. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2211926421000606?via%3Dihub#!
Počet záznamů: 1