Počet záznamů: 1
Harnessing subcellular-resolved organ distribution of cationic copolymer-functionalized fluorescent nanodiamonds for optimal delivery of active siRNA to a xenografted tumor in mice
- 1.
SYSNO ASEP 0542687 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Harnessing subcellular-resolved organ distribution of cationic copolymer-functionalized fluorescent nanodiamonds for optimal delivery of active siRNA to a xenografted tumor in mice Tvůrce(i) Claveau, S. (FR)
Kindermann, Marek (UOCHB-X)
Papine, A. (FR)
Díaz-Riascos, Z. V. (ES)
Délen, X. (FR)
Georges, P. (FR)
López-Alemany, R. (ES)
Tirado, O. M. (ES)
Bertrand, J.-R. (FR)
Abasolo, I. (ES)
Cígler, Petr (UOCHB-X) RID, ORCID
Treussart, F. (FR)Zdroj.dok. Nanoscale. - : Royal Society of Chemistry - ISSN 2040-3364
Roč. 13, č. 20 (2021), s. 9280-9292Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova protein corona ; drug-delivery ; cells Obor OECD Nano-materials (production and properties) CEP GA18-17071S GA ČR - Grantová agentura ČR EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_026/0008382 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CANAM II - 90056 - Ústav jaderné fyziky AV ČR, v. v. i. Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000649764500001 EID SCOPUS 85106949710 DOI 10.1039/d1nr00146a Anotace Diamond nanoparticles (nanodiamonds) can transport active drugs in cultured cells as well as in vivo. However, in the latter case, methods allowing the determination of their bioavailability accurately are still lacking. A nanodiamond can be made fluorescent with a perfectly stable emission and a lifetime ten times longer than that of tissue autofluorescence. Taking advantage of these properties, we present an automated quantification method of fluorescent nanodiamonds (FND) in histological sections of mouse organs and tumors, after systemic injection. We use a home-made time-delayed fluorescence microscope comprising a custom pulsed laser source synchronized on the master clock of a gated intensified array detector. This setup allows ultra-high-resolution images (120 Mpixels in size) of whole mouse organ sections to be obtained, with subcellular resolution and single-particle sensitivity. As a proof-of-principle experiment, we quantified the biodistribution and aggregation state of new cationic FNDs capable of transporting small interfering RNA inhibiting the oncogene responsible for Ewing sarcoma. Image analysis showed a low yield of nanodiamonds in the tumor after intravenous injection. Thus, for the in vivo efficacy assay, we injected the nanomedicine into the tumor. We achieved a 28-fold inhibition of the oncogene. This method can readily be applied to other nanoemitters with ≈100 ns lifetime. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2022 Elektronická adresa https://doi.org/10.1039/D1NR00146A
Počet záznamů: 1