Počet záznamů: 1
The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts
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SYSNO ASEP 0542427 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Effect of the Controlled Release of Platelet Lysate from PVA Nanomats on Keratinocytes, Endothelial Cells and Fibroblasts Tvůrce(i) Filová, Elena (FGU-C) RID, ORCID
Blanquer, Andreu (FGU-C) RID, ORCID
Knitlová, Jarmila (FGU-C) RID, ORCID
Plencner, Martin (FGU-C) RID, ORCID
Jenčová, V. (CZ)
Kopřivová, B. (CZ)
Lisnenko, M. (CZ)
Kuželová Košťáková, E. (CZ)
Procházková, R. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 995 Zdroj.dok. Nanomaterials. - : MDPI
Roč. 11, č. 4 (2021)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova controlled release ; platelet lysate ; PVA nanofibers ; endothelial cells ; keratinocytes ; fibroblasts ; cell differentiation Vědní obor RIV FB - Endokrinologie, diabetologie, metabolizmus, výživa Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP NV18-01-00332 GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000643386100001 EID SCOPUS 85104032421 DOI 10.3390/nano11040995 Anotace Platelet lysate (PL) provides a natural source of growth factors and other bioactive molecules, and the local controlled release of these bioactive PL components is capable of improving the healing of chronic wounds. Therefore, we prepared composite nanofibrous meshes via the needleless electrospinning technique using poly(vinyl alcohol) (PVA) with a high molecular weight and with a high degree of hydrolysis with the incorporated PL (10% w/w). The morphology, wettability and protein release from the nanofibers was then assessed from the resulting composite PVA-PL nanomats. The bioactivity of the PVA-PL nanomats was proved in vitro using HaCaT keratinocytes, human saphenous endothelial cells (HSVECs) and 3T3 fibroblasts. The PVA-PL supported cell adhesion, proliferation, and viability. The improved phenotypic maturation of the HaCaT cells due to the PVA-PL was manifested via the formation of intermediate filaments positive for cytokeratin 10. The PVA-PL enhanced both the synthesis of the von Willebrand factor via HSVECs and HSVECs chemotaxis through membranes with 8 mu m-sized pores. These results indicated the favorable effects of the PVA-PL nanomats on the three cell types involved in the wound healing process, and established PVA-PL nanomats as a promising candidate for further evaluation with respect to in vivo experiments. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2079-4991/11/4/995
Počet záznamů: 1