Počet záznamů: 1
Horizontal deformation of skeletal muscle thick sections visualised by confocal microscopy
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SYSNO ASEP 0542133 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Horizontal deformation of skeletal muscle thick sections visualised by confocal microscopy Tvůrce(i) Umek, N. (SI)
Janáček, Jiří (FGU-C) RID, ORCID
Cvetko, E. (SI)
Eržen, I. (SI)Zdroj.dok. Journal of Microscopy. - : Wiley - ISSN 0022-2720
Roč. 282, č. 2 (2021), s. 113-122Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova 3D ; fibre diameter ; horizontal dilation ; sample deformation ; shrinkage in Z direction Vědní obor RIV FP - Ostatní lékařské obory Obor OECD Anatomy and morphology (plant science to be 1.6) CEP LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000594502600001 EID SCOPUS 85096685198 DOI 10.1111/jmi.12985 Anotace Certain morphological parameters of the skeletal muscle tissue can be better understood via 3D considerations. Fluorescent confocal microscopy of thick tissue sections is a well-established method for visualising and measuring skeletal muscle fibres and surrounding capillaries in 3D. However, thick tissue sections are prone to deformations which may significantly influence some stereological and morphometric results like muscle fibre diameter and capillary length, but not dimensionless parameters like object number and Euler-Poincare characteristics. To better understand this phenomenon, we studied the horizontal deformation of thick (100 mu m) transverse skeletal muscle sections, by comparing the muscle fibre diameters measured on thick sections to muscle fibre diameters measured on thin (10 mu m) sections of the same sample. Diameter changes were further correlated with shrinkage in the Z direction (axial shrinkage) and deviation of the muscle fibre preferential axis from the Z-axis. We showed that the thick sections dilated in horizontal and shrunk in Z direction, and that the magnitude of horizontal dilation was associated with the magnitude of shrinkage in the Z direction. The latter was more pronounced in transversely than obliquely cut tissue sections. The results emphasise that even when shrinkage in the Z direction can be corrected using calibration, it is important to optimise histological protocols to minimise the Z-axis collapse that could cause horizontal dilation. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://doi.org/10.1111/jmi.12985
Počet záznamů: 1