Počet záznamů: 1  

Horizontal deformation of skeletal muscle thick sections visualised by confocal microscopy

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    SYSNO ASEP0542133
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevHorizontal deformation of skeletal muscle thick sections visualised by confocal microscopy
    Tvůrce(i) Umek, N. (SI)
    Janáček, Jiří (FGU-C) RID, ORCID
    Cvetko, E. (SI)
    Eržen, I. (SI)
    Zdroj.dok.Journal of Microscopy - ISSN 0022-2720
    Roč. 282, č. 2 (2021), s. 113-122
    Poč.str.10 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slova3D ; fibre diameter ; horizontal dilation ; sample deformation ; shrinkage in Z direction
    Vědní obor RIVFP - Ostatní lékařské obory
    Obor OECDAnatomy and morphology (plant science to be 1.6)
    CEPLM2018129 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    EF16_013/0001775 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOmezený přístup
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000594502600001
    EID SCOPUS85096685198
    DOI10.1111/jmi.12985
    AnotaceCertain morphological parameters of the skeletal muscle tissue can be better understood via 3D considerations. Fluorescent confocal microscopy of thick tissue sections is a well-established method for visualising and measuring skeletal muscle fibres and surrounding capillaries in 3D. However, thick tissue sections are prone to deformations which may significantly influence some stereological and morphometric results like muscle fibre diameter and capillary length, but not dimensionless parameters like object number and Euler-Poincare characteristics. To better understand this phenomenon, we studied the horizontal deformation of thick (100 mu m) transverse skeletal muscle sections, by comparing the muscle fibre diameters measured on thick sections to muscle fibre diameters measured on thin (10 mu m) sections of the same sample. Diameter changes were further correlated with shrinkage in the Z direction (axial shrinkage) and deviation of the muscle fibre preferential axis from the Z-axis. We showed that the thick sections dilated in horizontal and shrunk in Z direction, and that the magnitude of horizontal dilation was associated with the magnitude of shrinkage in the Z direction. The latter was more pronounced in transversely than obliquely cut tissue sections. The results emphasise that even when shrinkage in the Z direction can be corrected using calibration, it is important to optimise histological protocols to minimise the Z-axis collapse that could cause horizontal dilation.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2022
Počet záznamů: 1