Počet záznamů: 1
Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils
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SYSNO ASEP 0541648 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Differentiation of adipose tissue-derived stem cells towards vascular smooth muscle cells on modified poly(L-lactide) foils Tvůrce(i) Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Kasálková-Slepičková, N. (CZ)
Sedlář, Antonín (FGU-C) ORCID
Molitor, M. (CZ)
Musílková, Jana (FGU-C) RID, ORCID
Slepička, P. (CZ)
Švorčík, V. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 025016 Zdroj.dok. Biomedical Materials. - : Institute of Physics Publishing - ISSN 1748-6041
Roč. 16, č. 2 (2021)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Poly(L-lactic acid) (PLLA) ; plasma treatment ; dextran ; polyethylene glycol (PEG) ; adipose tissue-derived stem cells (ADSCs) ; differentiation ; vascular smooth muscle cells (VSMCs) Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA17-00885S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000619157900001 EID SCOPUS 85101595346 DOI 10.1088/1748-605X/abaf97 Anotace The aim of our research was to study the behaviour of adipose tissue-derived stem cells (ADSCs) and vascular smooth muscle cells (VSMCs) on variously modified poly(L-lactide) (PLLA) foils, namely on pristine PLLA, plasma-treated PLLA, PLLA grafted with polyethylene glycol (PEG), PLLA grafted with dextran (Dex), and the tissue culture polystyrene (PS) control. On these materials, the ADSCs were biochemically differentiated towards VSMCs by a medium supplemented with TGF beta 1, BMP4 and ascorbic acid (i.e. differentiation medium). ADSCs cultured in a non-differentiation medium were used as a negative control. Mature VSMCs cultured in both types of medium were used as a positive control. The impact of the variously modified PLLA foils and/or differences in the composition of the medium were studied with reference to cell adhesion, growth and differentiation. We observed similar adhesion and growth of ADSCs on all PLLA samples when they were cultured in the non-differentiation medium. The differentiation medium supported the expression of specific early, mid-term and/or late markers of differentiation (i.e. type I collagen, alpha SMA, calponin, smoothelin, and smooth muscle myosin heavy chain) in ADSCs on all tested samples. Moreover, ADSCs cultured in the differentiation medium revealed significant differences in cell growth among the samples that were similar to the differences observed in the cultures of VSMCs. The round morphology of the VSMCs indicated worse adhesion to pristine PLLA, and this sample was also characterized by the lowest cell proliferation. Culturing VSMCs in the differentiation medium inhibited their metabolic activity and reduced the cell numbers. Both cell types formed the most stable monolayer on plasma-treated PLLA and on the PS control. The behaviour of ADSCs and VSMCs on the tested PLLA foils differed according to the specific cell type and culture conditions. The suitable biocompatibility of both cell types on the tested PLLA foils seems to be favourable for vascular tissue engineering purposes. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://doi.org/10.1088/1748-605X/abaf97
Počet záznamů: 1