Počet záznamů: 1
Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation
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SYSNO ASEP 0541641 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Growth Factors VEGF-A(165) and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation Tvůrce(i) Sedlář, Antonín (FGU-C) ORCID
Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Matějka, Roman (FGU-C) RID, ORCID, SAI
Pražák, Šimon (FGU-C) ORCID
Mészáros, Zuzana (MBU-M)
Bojarová, Pavla (MBU-M) ORCID
Bačáková, Lucie (FGU-C) RID, ORCID
Křen, Vladimír (MBU-M) RID, ORCID
Slámová, Kristýna (MBU-M) RID, ORCIDČíslo článku 1843 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 4 (2021)Poč.str. 27 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova heterologous expression ; recombinant vascular endothelial growth factor (VEGF) ; basic fibroblast growth factor (bFGF) ; adult stem cells ; endothelial cells ; cell adhesion ; cell proliferation ; tissue engineering ; regenerative medicine ; vascular replacements Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) Vědní obor RIV – spolupráce Mikrobiologický ústav - Biochemie CEP GA18-01163S GA ČR - Grantová agentura ČR NV18-02-00422 GA MZd - Ministerstvo zdravotnictví LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LTC18038 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; MBU-M - RVO:61388971 UT WOS 000623786800001 EID SCOPUS 85100662467 DOI 10.3390/ijms22041843 Anotace Vascular endothelial growth factor-A(165) (VEGF-A(165)) and fibroblast growth factor-2 (FGF-2) are currently used for the functionalization of biomaterials designed for tissue engineering. We have developed a new simple method for heterologous expression and purification of VEGF-A(165) and FGF-2 in the yeast expression system of Pichia pastoris. The biological activity of the growth factors was assessed in cultures of human and porcine adipose tissue-derived stem cells (ADSCs) and human umbilical vein endothelial cells (HUVECs). When added into the culture medium, VEGF-A(165) stimulated proliferation only in HUVECs, while FGF-2 stimulated the proliferation of both cell types. A similar effect was achieved when the growth factors were pre-adsorbed to polystyrene wells. The effect of our recombinant growth factors was slightly lower than that of commercially available factors, which was attributed to the presence of some impurities. The stimulatory effect of the VEGF-A(165) on cell adhesion was rather weak, especially in ADSCs. FGF-2 was a potent stimulator of the adhesion of ADSCs but had no to negative effect on the adhesion of HUVECs. In sum, FGF-2 and VEGF-A(165) have diverse effects on the behavior of different cell types, which maybe utilized in tissue engineering. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/4/1843
Počet záznamů: 1