Počet záznamů: 1

Loss of COX4I1 Leads to Combined Respiratory Chain Deficiency and Impaired Mitochondrial Protein Synthesis

SYSNO ASEP	0541622
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Loss of COX4I1 Leads to Combined Respiratory Chain Deficiency and Impaired Mitochondrial Protein Synthesis
Tvůrce(i)	Čunátová, Kristýna (FGU-C)_{RID, ORCID} Pajuelo-Reguera, David (FGU-C)_{RID, ORCID, SAI} Vrbacký, Marek (FGU-C)_{RID, ORCID} Fernández-Vizarra, E. (GB) Ding, SJ. (GB) Fearnley, I.M. (GB) Zeviani, M. (GB) Houštěk, Josef (FGU-C)_{RID, ORCID} Mráček, Tomáš (FGU-C)_{RID, ORCID} Pecina, Petr (FGU-C)_{RID, ORCID}
Číslo článku	369
Zdroj.dok.	Cells. - : MDPI Roč. 10, č. 2 (2021)
Poč.str.	18 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	mitochondria ; OXPHOS ; cI ; COX ; cIV ; COX4 ; knock-out ; cIV assembly ; complex I ; biogenesis interdependency ; complexome profiling ; mitochondrial protein synthesis
Vědní obor RIV	CE - Biochemie
Obor OECD	Biochemistry and molecular biology
CEP	GA16-13671S GA ČR - Grantová agentura ČR
	NV19-07-00149 GA MZd - Ministerstvo zdravotnictví
	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000622356000001
EID SCOPUS	85101349220
DOI	10.3390/cells10020369
Anotace	The oxidative phosphorylation (OXPHOS) system localized in the inner mitochondrial membrane secures production of the majority of ATP in mammalian organisms. Individual OXPHOS complexes form supramolecular assemblies termed supercomplexes. The complexes are linked not only by their function but also by interdependency of individual complex biogenesis or maintenance. For instance, cytochrome c oxidase (cIV) or cytochrome bc1 complex (cIII) deficiencies affect the level of fully assembled NADH dehydrogenase (cI) in monomeric as well as supercomplex forms. It was hypothesized that cI is affected at the level of enzyme assembly as well as at the level of cI stability and maintenance. However, the true nature of interdependency between cI and cIV is not fully understood yet. We used a HEK293 cellular model where the COX4 subunit was completely knocked out, serving as an ideal system to study interdependency of cI and cIV, as early phases of cIV assembly process were disrupted. Total absence of cIV was accompanied by profound deficiency of cI, documented by decrease in the levels of cI subunits and significantly reduced amount of assembled cI. Supercomplexes assembled from cI, cIII, and cIV were missing in COX4I1 knock-out (KO) due to loss of cIV and decrease in cI amount. Pulse-chase metabolic labeling of mitochondrial DNA (mtDNA)-encoded proteins uncovered a decrease in the translation of cIV and cI subunits. Moreover, partial impairment of mitochondrial protein synthesis correlated with decreased content of mitochondrial ribosomal proteins. In addition, complexome profiling revealed accumulation of cI assembly intermediates, indicating that cI biogenesis, rather than stability, was affected. We propose that attenuation of mitochondrial protein synthesis caused by cIV deficiency represents one of the mechanisms, which may impair biogenesis of cI.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2022
Elektronická adresa	https://www.mdpi.com/2073-4409/10/2/369

Počet záznamů: 1