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Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats

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    SYSNO ASEP0541546
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevSpinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats
    Tvůrce(i) Mrózková, Petra (FGU-C) RID, ORCID
    Špicarová, Diana (FGU-C) RID, ORCID
    Paleček, Jiří (FGU-C) RID, ORCID
    Číslo článku991
    Zdroj.dok.International Journal of Molecular Sciences. - : MDPI
    Roč. 22, č. 3 (2021)
    Poč.str.16 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovaPAR2 ; TRPV1 ; synaptic transmission ; superficial dorsal horn ; spinal cord ; nociception ; peripheral inflammation ; thermal hyperalgesia ; inflammatory pain
    Vědní obor RIVFH - Neurologie, neurochirurgie, neurovědy
    Obor OECDNeurosciences (including psychophysiology
    CEPGA18-09853S GA ČR - Grantová agentura ČR
    GA20-19136S GA ČR - Grantová agentura ČR
    ED1.1.00/02.0109 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000615302300001
    EID SCOPUS85099703025
    DOI10.3390/ijms22030991
    AnotaceThe mechanisms of inflammatory pain need to be identified in order to find new superior treatments. Protease-activated receptors 2 (PAR2) and transient receptor potential vanilloid 1 (TRPV1) are highly co-expressed in dorsal root ganglion neurons and implicated in pain development. Here, we examined the role of spinal PAR2 in hyperalgesia and the modulation of synaptic transmission in carrageenan-induced peripheral inflammation, using intrathecal (i.t.) treatment in the behavioral experiments and recordings of spontaneous, miniature and dorsal root stimulation-evoked excitatory postsynaptic currents (sEPSCs, mEPSCs and eEPSCs) in spinal cord slices. Intrathecal PAR2-activating peptide (AP) administration aggravated the carrageenan-induced thermal hyperalgesia, and this was prevented by a TRPV1 antagonist (SB 366791) and staurosporine i.t. pretreatment. Additionally, the frequency of the mEPSC and sEPSC and the amplitude of the eEPSC recorded from the superficial dorsal horn neurons were enhanced after acute PAR2 AP application, while prevented with SB 366791 or staurosporine pretreatment. PAR2 antagonist application reduced the thermal hyperalgesia and decreased the frequency of mEPSC and sEPSC and the amplitude of eEPSC. Our findings highlight the contribution of spinal PAR2 activation to carrageenan-induced hyperalgesia and the importance of dorsal horn PAR2 and TRPV1 receptor interactions in the modulation of nociceptive synaptic transmission.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2022
    Elektronická adresahttps://www.mdpi.com/1422-0067/22/3/991
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