Počet záznamů: 1

Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats

SYSNO ASEP	0541546
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Spinal PAR2 Activation Contributes to Hypersensitivity Induced by Peripheral Inflammation in Rats
Tvůrce(i)	Mrózková, Petra (FGU-C)_{RID, ORCID} Špicarová, Diana (FGU-C)_{RID, ORCID} Paleček, Jiří (FGU-C)_{RID, ORCID}
Číslo článku	991
Zdroj.dok.	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 3 (2021)
Poč.str.	16 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	PAR2 ; TRPV1 ; synaptic transmission ; superficial dorsal horn ; spinal cord ; nociception ; peripheral inflammation ; thermal hyperalgesia ; inflammatory pain
Vědní obor RIV	FH - Neurologie, neurochirurgie, neurovědy
Obor OECD	Neurosciences (including psychophysiology
CEP	GA18-09853S GA ČR - Grantová agentura ČR
	GA20-19136S GA ČR - Grantová agentura ČR
	ED1.1.00/02.0109 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000615302300001
EID SCOPUS	85099703025
DOI	10.3390/ijms22030991
Anotace	The mechanisms of inflammatory pain need to be identified in order to find new superior treatments. Protease-activated receptors 2 (PAR2) and transient receptor potential vanilloid 1 (TRPV1) are highly co-expressed in dorsal root ganglion neurons and implicated in pain development. Here, we examined the role of spinal PAR2 in hyperalgesia and the modulation of synaptic transmission in carrageenan-induced peripheral inflammation, using intrathecal (i.t.) treatment in the behavioral experiments and recordings of spontaneous, miniature and dorsal root stimulation-evoked excitatory postsynaptic currents (sEPSCs, mEPSCs and eEPSCs) in spinal cord slices. Intrathecal PAR2-activating peptide (AP) administration aggravated the carrageenan-induced thermal hyperalgesia, and this was prevented by a TRPV1 antagonist (SB 366791) and staurosporine i.t. pretreatment. Additionally, the frequency of the mEPSC and sEPSC and the amplitude of the eEPSC recorded from the superficial dorsal horn neurons were enhanced after acute PAR2 AP application, while prevented with SB 366791 or staurosporine pretreatment. PAR2 antagonist application reduced the thermal hyperalgesia and decreased the frequency of mEPSC and sEPSC and the amplitude of eEPSC. Our findings highlight the contribution of spinal PAR2 activation to carrageenan-induced hyperalgesia and the importance of dorsal horn PAR2 and TRPV1 receptor interactions in the modulation of nociceptive synaptic transmission.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2022
Elektronická adresa	https://www.mdpi.com/1422-0067/22/3/991

Počet záznamů: 1