Počet záznamů: 1
3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice
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SYSNO ASEP 0541406 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název 3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice Tvůrce(i) Děd, Lukáš (BTO-N) RID
Hwang, J.Y. (US)
Miki, K. (US)
Shi, H. F. (US)
Chung, J.-J. (US)Celkový počet autorů 5 Číslo článku e62043 Zdroj.dok. eLife. - : eLife - ISSN 2050-084X
Roč. 9, OCT 20 2020 (2020)Poč.str. 29 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Sperm Capacitation ; Acrosome Reaction ; Spermatozoa Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000595597500001 EID SCOPUS 85095704918 DOI 10.7554/eLife.62043 Anotace Out of millions of ejaculated sperm, a few reach the fertilization site in mammals. Flagellar Ca2+ signaling nanodomains, organized by multi-subunit CatSper calcium channel complexes, are pivotal for sperm migration in the female tract, implicating CatSper-dependent mechanisms in sperm selection. Here using biochemical and pharmacological studies, we demonstrate that CatSper1 is an O-linked glycosylated protein, undergoing capacitation-induced processing dependent on Ca2+ and phosphorylation cascades. CatSper1 processing correlates with protein tyrosine phosphorylation (pY) development in sperm cells capacitated in vitro and in vivo. Using 3D in situ molecular imaging and ANN-based automatic detection of sperm distributed along the cleared female tract, we demonstrate that spermatozoa past the utero-tubal junction possess the intact CatSper1 signals. Together, we reveal that fertilizing mouse spermatozoa in situ are characterized by intact CatSper channel, lack of pY, and reacted acrosomes. These findings provide molecular insight into sperm selection for successful fertilization in the female reproductive tract. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2021 Elektronická adresa https://elifesciences.org/articles/62043
Počet záznamů: 1