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Functionalization of polymeric nanofibers using platelets for melanocyte culture
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SYSNO ASEP 0539763 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název Functionalization of polymeric nanofibers using platelets for melanocyte culture Tvůrce(i) Vocetková, Karolína (UEM-P) ORCID
Sovková, Věra (UEM-P) ORCID
Buzgo, Matej (UEM-P)
Divín, Radek (UEM-P)
Amler, Evžen (UEM-P) RID
Filová, Eva (UEM-P) RID, ORCIDZdroj.dok. Lékař a technika. Biomedicinské inženýrství a informatika. - : Česká lékařská společnost J. E. Purkyně - ISSN 0301-5491
Roč. 50, č. 1 (2020), s. 16-22Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova nanofibers ; platelets ; tissue engineering Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) CEP LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 EID SCOPUS 85092597508 DOI 10.14311/CTJ.2020.1.03 Anotace Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the therapy of multiple diseases, including depigmentation disorders. The most common depigmentation disorder is vitiligo, a disease with deep psychosocial implications. Thanks to their unique properties, electrospun polymeric nanofibers represent a material suitable for tissue engineering applications. Furthermore, they may be functionalized with platelets, cells that contain a wide spectrum of growth factors and chemokines. The aim of this paper was to evaluate the functionalization of polymeric nanofibers with platelets and their effects in melanocyte culture. The scaffolds were visualized using scanning electron microscopy, the metabolic activity and proliferation of melanocytes was determined using MTS assay and dsDNA quantification, respectively. Furthermore, the melanocytes were stained and visualized using confocal microscopy. The acquired data showed that poly-ε-caprolactone functionalized with platelets promoted the viability and proliferation of melanocytes. According to the results, such a functionalized scaffold combining nanofibers and platelets may be suitable for melanocyte culture. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2021 Elektronická adresa https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/6131
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