Počet záznamů: 1  

Single-Cell RNA Sequencing Unravels Heterogeneity of the Stromal Niche in Cutaneous Melanoma Heterogeneous Spheroids

  1. 1.
    SYSNO ASEP0539639
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevSingle-Cell RNA Sequencing Unravels Heterogeneity of the Stromal Niche in Cutaneous Melanoma Heterogeneous Spheroids
    Tvůrce(i) Novotný, Jiří (UMG-J)
    Strnadová, K. (CZ)
    Dvořánková, B. (CZ)
    Kocourková, Šárka (UMG-J)
    Jaksa, R. (CZ)
    Dundr, P. (CZ)
    Pačes, Václav (UMG-J) RID
    Smetana, K. (CZ)
    Kolář, Michal (UMG-J) RID
    Lacina, L. (CZ)
    Celkový počet autorů10
    Číslo článku3324
    Zdroj.dok.Cancers (Basel). - : MDPI
    Roč. 12, č. 11 (2020)
    Poč.str.21 s.
    Forma vydáníOnline - E
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovamelanoma ; spheroids ; fibroblasts ; subpopulation ; heterogeneity ; Interleukin-6 ; cytokine ; senescence-associated secretory phenotype ; extracellular matrix ; single-cell sequencing
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDBiochemistry and molecular biology
    CEPLM2018131 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaUMG-J - RVO:68378050
    UT WOS000592838500001
    DOI10.3390/cancers12113324
    AnotaceHeterogeneous spheroids have recently acquired a prominent position in melanoma research because they incorporate microenvironmental cues relevant for melanoma. In this study, we focused on the analysis of microenvironmental factors introduced in melanoma heterogeneous spheroids by different dermal fibroblasts. We aimed to map the fibroblast diversity resulting from previously acquired damage caused by exposure to extrinsic and intrinsic stimuli. To construct heterogeneous melanoma spheroids, we used normal dermal fibroblasts from the sun-protected skin of a juvenile donor. We compared them to the fibroblasts from the sun-exposed photodamaged skin of an adult donor. Further, we analysed the spheroids by single-cell RNA sequencing. To validate transcriptional data, we also compared the immunohistochemical analysis of heterogeneous spheroids to melanoma biopsies. We have distinguished three functional clusters in primary human fibroblasts from melanoma spheroids. These clusters differed in the expression of (a) extracellular matrix-related genes, (b) pro-inflammatory factors, and (c) TGFβ signalling superfamily. We observed a broader deregulation of gene transcription in previously photodamaged cells. We have confirmed that pro-inflammatory cytokine IL-6 significantly enhances melanoma invasion to the extracellular matrix in our model. This supports the opinion that the aspects of ageing are essential for reliable melanoma 3D modelling in vitro.
    PracovištěÚstav molekulární genetiky
    KontaktGabriela Marešová, maresova@img.cas.cz, Tel.: 241 063 217
    Rok sběru2021
    Elektronická adresahttps://www.mdpi.com/2072-6694/12/11/3324
Počet záznamů: 1