Počet záznamů: 1
Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system
- 1.
SYSNO ASEP 0537554 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Histone post-translational modifications in Silene latifolia X and Y chromosomes suggest a mammal-like dosage compensation system Tvůrce(i) Rodriguez Lorenzo, José Luis (BFU-R)
Hubinský, Marcel (BFU-R)
Vyskot, Boris (BFU-R) RID, ORCID
Hobza, Roman (BFU-R) RID, ORCIDCelkový počet autorů 4 Číslo článku 110528 Zdroj.dok. Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 299, č. 2020 (2020)Poč.str. 7 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. IE - Irsko Klíč. slova sex-chromosomes ; dna methylation ; dioecious plant ; recombination suppression ; repetitive sequences ; evolution ; degeneration Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA18-06147S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000569805800011 EID SCOPUS 85089176013 DOI 10.1016/j.plantsci.2020.110528 Anotace Silene latifolia is a model organism to study evolutionary young heteromorphic sex chromosome evolution in plants. Previous research indicates a Y-allele gene degeneration and a dosage compensation system already operating. Here, we propose an epigenetic approach based on analysis of several histone post-translational modifications (PTMs) to find the first epigenetic hints of the X:Y sex chromosome system regulation in S. latifolia. Through chromatin immunoprecipitation we interrogated six genes from X and Y alleles. Several histone PTMS linked to DNA methylation and transcriptional repression (H3K27me3, H3K23me, H3K9me2 and H3K9me3) and to transcriptional activation (H3K4me3 and H4K5, 8, 12, 16ac) were used. DNA enrichment (Immunoprecipitated DNA/input DNA) was analyzed and showed three main results: (i) promoters of the Y allele are associated with heterochromatin marks, (ii) promoters of the X allele in males are associated with activation of transcription marks and finally, (iii) promoters of X alleles in females are associated with active and repressive marks. Our finding indicates a transcription activation of X allele and transcription repression of Y allele in males. In females we found a possible differential regulation (up X-1, down X-2) of each female X allele. These results agree with the mammal-like epigenetic dosage compensation regulation. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0168945220301333?via%3Dihub
Počet záznamů: 1