Počet záznamů: 1

Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties

SYSNO ASEP	0536963
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Separation of the glycosylated carotenoid myxoxanthophyll from synechocystis salina by hpccc and evaluation of its antioxidant, tyrosinase inhibitory and immune-stimulating properties
Tvůrce(i)	Nováková, Michaela (MBU-M) Fábryová, Tereza (MBU-M)_ORCID Vokurková, D. (CZ) Dolečková, I. (CZ) Kopecký, Jiří (MBU-M)_ORCID Hrouzek, Pavel (MBU-M)_ORCID Tůmová, L. (CZ) Cheel, José (MBU-M)_{RID, ORCID}
Číslo článku	73
Zdroj.dok.	Separations. - : MDPI Roč. 7, č. 4 (2020)
Poč.str.	15 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	Centrifugal partition chromatography (CPC) ; Countercurrent chromatography (CCC) ; Cyanobacteria ; High-performance countercurrent chromatography (HPCCC) ; Myxoxanthophyll
Vědní obor RIV	EE - Mikrobiologie, virologie
Obor OECD	Microbiology
CEP	LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000601724400001
EID SCOPUS	85097942310
DOI	10.3390/separations7040073
Anotace	Global demand for natural pigments has increased in the past few years. Myxoxanthophyll, a glycosylated monocyclic carotenoid, is a pigment that occurs naturally in cyanobacteria but no scalable isolation process has been developed to obtain it from its natural source to date. In this study, myxoxanthophyll was isolated from unicellular cyanobacterium Synechocystis salina (S. salina) using high-performance countercurrent chromatography (HPCCC), where the lower phase of the biphasic solvent system composed of n-heptane–ethanol–water (2:4:4, v/v/v) was used as a mobile phase, whereas its upper phase was the stationary phase. For the HPCCC isolation, a multi-injection method was developed, and four consecutive sample injections (70 mg each) were performed, obtaining, in total, 20 mg of myxoxanthophyll, which was finally purified with high-performance liquid chromatography (HPLC). Overall, a final myxoxanthophyll yield of 15 mg (98% purity) was obtained. The target pigment showed a weak antioxidant and tyrosinase inhibitory effect, and exhibited immune-stimulating properties by activating human granulocytes. The results presented here form a basis for the large-scale production of myxoxanthophyll, and show the potential benefits of this pigment for human health.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2021
Elektronická adresa	https://www.mdpi.com/2297-8739/7/4/73

Počet záznamů: 1