Počet záznamů: 1
Simultaneous presence of bacteriochlorophyll and xanthorhodopsin genes in a freshwater bacterium
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SYSNO ASEP 0536958 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Simultaneous presence of bacteriochlorophyll and xanthorhodopsin genes in a freshwater bacterium Tvůrce(i) Kopejtka, Karel (MBU-M) ORCID, RID
Tomasch, J. (DE)
Zeng, Yonghui (MBU-M) ORCID
Selyanin, Vadim (MBU-M) RID
Dachev, Marko (MBU-M) ORCID
Piwosz, Kasia (MBU-M) ORCID
Tichý, Martin (MBU-M) RID
Bína, David (BC-A) RID, ORCID
Gardian, Zdenko (BC-A) RID
Bunk, B. (DE)
Brinkmann, H. (DE)
Geffers, R. (DE)
Sommaruga, R. (AT)
Koblížek, Michal (MBU-M) RID, ORCIDČíslo článku e01044-20 Zdroj.dok. mSystems. - : American Society for Microbiology - ISSN 2379-5077
Roč. 5, č. 6 (2020)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Aerobic anoxygenic phototrophic bacteria ; Bacteriochlorophyll ; Gene expression ; Photosynthesis gene cluster ; Rhodopsin Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Fyziologie CEP GJ18-14095Y GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000630974900017 EID SCOPUS 85098211480 DOI 10.1128/mSystems.01044-20 Anotace Photoheterotrophic bacteria represent an important part of aquatic microbial communities. There exist two fundamentally different light-harvesting systems: bacteriochlorophyll-containing reaction centers or rhodopsins. Here, we report a photoheterotrophic Sphingomonas strain isolated from an oligotrophic lake, which contains complete sets of genes for both rhodopsin-based and bacteriochlorophyllbased phototrophy. Interestingly, the identified genes were not expressed when cultured in liquid organic media. Using reverse transcription quantitative PCR (RT-qPCR), RNA sequencing, and bacteriochlorophyll a quantification, we document that bacteriochlorophyll synthesis was repressed by high concentrations of glucose or galactose in the medium. Coactivation of photosynthesis genes together with genes for TonBdependent transporters suggests the utilization of light energy for nutrient import. The photosynthetic units were formed by ring-shaped light-harvesting complex 1 and reaction centers with bacteriochlorophyll a and spirilloxanthin as the main lightharvesting pigments. The identified rhodopsin gene belonged to the xanthorhodopsin family, but it lacks salinixanthin antenna. In contrast to bacteriochlorophyll, the expression of xanthorhodopsin remained minimal under all experimental conditions tested. Since the gene was found in the same operon as a histidine kinase, we propose that it might serve as a light sensor. Our results document that photoheterotrophic Sphingomonas bacteria use the energy of light under carbon-limited conditions, while under carbon-replete conditions, they cover all their metabolic needs through oxidative phosphorylation. IMPORTANCE Phototrophic organisms are key components of many natural environments. There exist two main phototrophic groups: species that collect light energy using various kinds of (bacterio)chlorophylls and species that utilize rhodopsins Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://msystems.asm.org/content/5/6/e01044-20
Počet záznamů: 1