Počet záznamů: 1
Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes
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SYSNO ASEP 0536178 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Tail-Oxidized Cholesterol Enhances Membrane Permeability for Small Solutes Tvůrce(i) Olžyńska, Agnieszka (UFCH-W) RID
Kulig, W. (FI)
Mikkolainen, H. (FI)
Czerniak, T. (PL)
Jurkiewicz, Piotr (UFCH-W) RID, ORCID
Cwiklik, Lukasz (UFCH-W) RID, ORCID
Rog, T. (FI)
Hof, Martin (UFCH-W) RID, ORCID
Jungwirth, Pavel (UOCHB-X) RID, ORCID
Vattulainen, I. (FI)Zdroj.dok. Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 36, č. 35 (2020), s. 10438-10447Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova molecular-dynamics simulations ; alkyl chain unsaturation ; atom force-field ; lipid-bilayer ; phospholipids ; liposomes ; oxidation ; translocation ; transitions ; oxysterols Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry Vědní obor RIV – spolupráce Ústav organické chemie a biochemie - Fyzikální chemie a teoretická chemie CEP GX19-26854X GA ČR - Grantová agentura ČR GA18-26751S GA ČR - Grantová agentura ČR GA17-06792S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 ; UOCHB-X - RVO:61388963 UT WOS 000571389200016 EID SCOPUS 85090510741 DOI 10.1021/acs.langmuir.0c01590 Anotace Cholesterol renders mammalian cell membranes more compact by reducing the amount of voids in the membrane structure. Because of this, cholesterol is known to regulate the ability of cell membranes to prevent the permeation of water and water-soluble molecules through the membranes. Meanwhile, it is also known that even seemingly tiny modifications in the chemical structure of cholesterol can lead to notable changes in membrane properties. The question is, how significantly do these small changes in cholesterol structure affect the permeability barrier function of cell membranes? In this work, we applied fluorescence methods as well as atomistic molecular dynamics simulations to characterize changes in lipid membrane permeability induced by cholesterol oxidation. The studied 7 beta-hydroxycholesterol (7 beta-OH-chol) and 27-hydroxycholesterol (27-OH-chol) represent two distinct groups of oxysterols, namely, ring- and tail-oxidized cholesterols, respectively. Our previous research showed that the oxidation of the cholesterol tail has only a marginal effect on the structure of a lipid bilayer, however, oxidation was found to disturb membrane dynamics by introducing a mechanism that allows sterol molecules to move rapidly back and forth across the membranebobbing. Herein, we show that bobbing of 27-OH-chol accelerates fluorescence quenching of NBD-lipid probes in the inner leaflet of liposomes by dithionite added to the liposomal suspension. Systematic experiments using fluorescence quenching spectroscopy and microscopy led to the conclusion that the presence of 27-OH-chol increases membrane permeability to the dithionite anion. Atomistic molecular dynamics simulations demonstrated that 27-OH-chol also facilitates water transport across the membrane. The results support the view that oxysterol bobbing gives rise to successive perturbations to the hydrophobic core of the membrane, and these perturbations promote the permeation of water and small water-soluble molecules through a lipid bilayer. The observed impairment of permeability can have important consequences for eukaryotic organisms. The effects described for 27-OH-chol were not observed for 7 beta-OH-chol which represents ring-oxidized sterols. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2021 Elektronická adresa http://hdl.handle.net/11104/0313993
Počet záznamů: 1