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In vitro and in vivo testing of nanofibrous membranes doped with alaptide and L-arginine for wound treatment

SYSNO ASEP	0535787
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	In vitro and in vivo testing of nanofibrous membranes doped with alaptide and L-arginine for wound treatment
Tvůrce(i)	Mikeš, P. (CZ) Brož, Antonín (FGU-C)_{RID, ORCID, SAI} Sinica, A. (CZ) Asatiani, N. (CZ) Bačáková, Lucie (FGU-C)_{RID, ORCID}
Číslo článku	065023
Zdroj.dok.	Biomedical Materials. - : Institute of Physics Publishing - ISSN 1748-6041 Roč. 15, č. 6 (2020)
Poč.str.	18 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	wound dressing ; alaptide ; L-arginine ; electrospinning ; polycaprolactone ; nanofibers ; skin healing
Vědní obor RIV	JJ - Ostatní materiály
Obor OECD	Nano-processes (applications on nano-scale)
CEP	GA17-02448S GA ČR - Grantová agentura ČR
Způsob publikování	Omezený přístup
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000592335600001
EID SCOPUS	85096886043
DOI	10.1088/1748-605X/ab950f
Anotace	We have prepared a candidate biocompatible construct for skin wound healing based on electrospun polycaprolactone (PCL) nanofibrous membranes. The membrane material was loaded either with L-arginine or with alaptide, or with a mixture of both bioactive components. Alaptide is a spirocyclic synthetic dipeptide, an analogue of melanocyte-stimulating hormone release-inhibiting factor. L-arginine is an amino acid with a basic guanidine side chain. It is a direct precursor of nitric oxide, which plays a pivotal role in skin repair. The presence and the distribution of the additives were proved with high-performance liquid chromatography, Fourier-transform infrared spectroscopy and Raman spectroscopy. The influence of L-arginine and alaptide on the morphology of the membrane was characterized using scanning electron microscopy. No statistically significant correlation between fiber diameter and drug concentration was observed. The membranes were then tested in vitro for their cytotoxicity, using primary human dermal fibroblasts, in order to obtain the optimal concentrations of the additives for in vivo tests in a rat model. The membranes with the highest concentration of L-arginine (10 wt. %) proved to be cytotoxic. The membranes with alaptide in concentrations from 0.1 to 2.5 wt.%, and with the other L-arginine concentrations (1 and 5 wt.%), did not show high toxicity. In addition, there was no observed improvement in cell proliferation on the membranes. The in vivo experiments revealed that membranes with 1.5 wt.% of alaptide or with 1.5 wt.% of alaptide in combination with 5 wt.% of L-arginine markedly accelerated the healing of skin incisions, and particularly the healing of skin burns, i.e. wounds of relatively large extent. These results indicate that our newly-developed nanofibrous membranes are promising for treating wounds with large damaged areas, where a supporting material is needed.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2021
Elektronická adresa	https://doi.org/10.1088/1748-605X/ab950f

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