Počet záznamů: 1  

Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

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    SYSNO ASEP0535590
    Druh ASEPC - Konferenční příspěvek (mezinárodní konf.)
    Zařazení RIVD - Článek ve sborníku
    NázevSurface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring
    Tvůrce(i) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
    Ježek, Jan (UPT-D) RID, ORCID, SAI
    Kizovský, Martin (UPT-D)
    Klementová, Tereza (UPT-D) ORCID, SAI
    Krátký, Stanislav (UPT-D) RID, ORCID, SAI
    Sobota, Jaroslav (UPT-D) RID, ORCID, SAI
    Samek, Ota (UPT-D) RID, ORCID, SAI
    Zemánek, Pavel (UPT-D) RID, SAI, ORCID
    Buryška, T. (CZ)
    Damborský, J. (CZ)
    Prokop, Z. (CZ)
    Celkový počet autorů11
    Zdroj.dok.PhotonIcs and Electromagnetics Research Symposium - Spring, PIERS-Spring 2019. Proceedings. - New York : IEEE, 2019 - ISSN 1559-9450 - ISBN 978-1-7281-3403-1
    Rozsah strans. 1301-1309
    Poč.str.9 s.
    Forma vydáníTištěná - P
    AkcePhotonIcs and Electromagnetics Research Symposium - Spring (PIERS-Spring) 2019
    Datum konání17.06.2019 - 20.06.2019
    Místo konáníRome
    ZeměIT - Itálie
    Typ akceWRD
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovachemical analysis ; Raman spectroscopy ; emission spectroscopy ; light transmission
    Vědní obor RIVBH - Optika, masery a lasery
    Obor OECDOptics (including laser optics and quantum optics)
    CEPGA16-07965S GA ČR - Grantová agentura ČR
    LO1212 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    ED0017/01/01 GA MŠk - Ministerstvo školství, mládeže a tělovýchovy
    Institucionální podporaUPT-D - RVO:68081731
    UT WOS000550769301050
    EID SCOPUS85082018253
    AnotaceOptofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
    PracovištěÚstav přístrojové techniky
    KontaktMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Rok sběru2021
Počet záznamů: 1